Transcription enhances intrachromosomal homologous recombination in mammalian cells - PubMed (original) (raw)

Transcription enhances intrachromosomal homologous recombination in mammalian cells

J A Nickoloff. Mol Cell Biol. 1992 Dec.

Abstract

The influence of transcription on homologous intrachromosomal recombination between direct and inverted repeats has been examined by using Chinese hamster ovary cells. Recombination was monitored between two integrated neomycin (neo) genes, including one silent allele and a second allele regulated by the inducible mouse mammary tumor virus promoter. Transcription of mouse mammary tumor virus neo alleles was regulated with the glucocorticoid hormone dexamethasone. Alleles transcribed at high levels recombined about two- to sevenfold more frequently than identical alleles transcribed at low levels. Direct repeats recombined primarily by a gene conversion mechanism; inverted repeats produced a variety of rearranged products. These results are discussed in relation to recombinational processes that regulate gene expression, influence gene family structures, and mediate genomic instability associated with cellular transformation and tumorigenesis.

PubMed Disclaimer

Similar articles

• Chromosomal double-strand breaks induce gene conversion at high frequency in mammalian cells.
  Taghian DG, Nickoloff JA. Taghian DG, et al. Mol Cell Biol. 1997 Nov;17(11):6386-93. doi: 10.1128/MCB.17.11.6386. Mol Cell Biol. 1997. PMID: 9343400 Free PMC article.
• Transcription stimulates homologous recombination in mammalian cells.
  Nickoloff JA, Reynolds RJ. Nickoloff JA, et al. Mol Cell Biol. 1990 Sep;10(9):4837-45. doi: 10.1128/mcb.10.9.4837-4845.1990. Mol Cell Biol. 1990. PMID: 2167441 Free PMC article.
• Preferential repair of UV damage in highly transcribed DNA diminishes UV-induced intrachromosomal recombination in mammalian cells.
  Deng WP, Nickoloff JA. Deng WP, et al. Mol Cell Biol. 1994 Jan;14(1):391-9. doi: 10.1128/mcb.14.1.391-399.1994. Mol Cell Biol. 1994. PMID: 8264606 Free PMC article.
• Regulation of mouse mammary tumor virus gene expression by glucocorticoid hormones.
  Ringold GM. Ringold GM. Curr Top Microbiol Immunol. 1983;106:79-103. doi: 10.1007/978-3-642-69357-1_4. Curr Top Microbiol Immunol. 1983. PMID: 6315308 Review. No abstract available.
• Mouse mammary tumour virus: a proviral gene contributes to the understanding of eukaryotic gene expression and mammary tumorigenesis.
  Ponta H, Günzburg WH, Salmons B, Groner B, Herrlich P. Ponta H, et al. J Gen Virol. 1985 May;66 ( Pt 5):931-43. doi: 10.1099/0022-1317-66-5-931. J Gen Virol. 1985. PMID: 2987402 Review. No abstract available.

Cited by

• Transgenic mice harboring direct repeat substrates reveal key underlying causes of homologous recombination in vivo.
  Moise AC, Kay JE, Engelward BP. Moise AC, et al. DNA Repair (Amst). 2022 Dec;120:103419. doi: 10.1016/j.dnarep.2022.103419. Epub 2022 Oct 10. DNA Repair (Amst). 2022. PMID: 36257175 Free PMC article. Review.
• The UVSSA complex alleviates MYC-driven transcription stress.
  Sato M, Liebau RC, Liu Z, Liu L, Rabadan R, Gautier J. Sato M, et al. J Cell Biol. 2021 Feb 1;220(2):e201807163. doi: 10.1083/jcb.201807163. J Cell Biol. 2021. PMID: 33404608 Free PMC article.
• Engineering of α-PD-1 antibody-expressing long-lived plasma cells by CRISPR/Cas9-mediated targeted gene integration.
  Luo B, Zhan Y, Luo M, Dong H, Liu J, Lin Y, Zhang J, Wang G, Verhoeyen E, Zhang Y, Zhang H. Luo B, et al. Cell Death Dis. 2020 Nov 12;11(11):973. doi: 10.1038/s41419-020-03187-1. Cell Death Dis. 2020. PMID: 33184267 Free PMC article.
• Transcribing malignancy: transcription-associated genomic instability in cancer.
  Boulianne B, Feldhahn N. Boulianne B, et al. Oncogene. 2018 Feb 22;37(8):971-981. doi: 10.1038/onc.2017.402. Epub 2017 Nov 6. Oncogene. 2018. PMID: 29106394 Review.
• Transcription-replication conflicts at chromosomal fragile sites-consequences in M phase and beyond.
  Oestergaard VH, Lisby M. Oestergaard VH, et al. Chromosoma. 2017 Mar;126(2):213-222. doi: 10.1007/s00412-016-0617-2. Epub 2016 Oct 28. Chromosoma. 2017. PMID: 27796495 Review.

References

1. 1. Mol Cell Biol. 1984 Jun;4(6):1020-34 - PubMed
2. 1. Cell. 1981 Aug;25(2):517-24 - PubMed
3. 1. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2879-83 - PubMed
4. 1. Mol Cell Biol. 1991 Apr;11(4):1813-9 - PubMed
5. 1. Mol Cell Biol. 1991 Jan;11(1):289-98 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central
  • Taylor & Francis

• Other Literature Sources

  • The Lens - Patent Citations