Transposase-induced excision and circularization of the bacterial insertion sequence IS911 - PubMed (original) (raw)
Transposase-induced excision and circularization of the bacterial insertion sequence IS911
P Polard et al. EMBO J. 1992 Dec.
Abstract
We have investigated the role of three IS911-specified proteins in transposition in vivo: the products of the upstream (OrfA) and downstream (OrfB) open reading frames, and a transframe protein (OrfAB) produced by -1 translational frameshifting between orfA and orfB. The production of OrfAB alone is shown to lead both to excision and to circularization of the element and to be sufficient for intermolecular transposition into a plasmid target. Simultaneous and independent production of OrfA is shown to stimulate OrfAB-mediated intermolecular transposition while greatly reducing the appearance of transposon circles. We have not been able to detect a role for OrfB. Although under certain conditions, the vector plasmid undergoes precise resealing after IS911 excision, the data suggest that this is not normally the case and that the donor plasmid is not generally conserved. The use of IS911 derivatives carrying mutations in the terminal 2 bp suggested that circle formation represents a site-specific intramolecular transposition event. We present a model which explains both intra- and intermolecular transposition events in terms of a single reaction mechanism of the 'cut and paste' type.
Similar articles
- Efficient transposition of IS911 circles in vitro.
Ton-Hoang B, Polard P, Chandler M. Ton-Hoang B, et al. EMBO J. 1998 Feb 16;17(4):1169-81. doi: 10.1093/emboj/17.4.1169. EMBO J. 1998. PMID: 9463394 Free PMC article. - Inhibition of transpositional recombination by OrfA and OrfB proteins encoded by insertion sequence IS3.
Sekine Y, Izumi K, Mizuno T, Ohtsubo E. Sekine Y, et al. Genes Cells. 1997 Sep;2(9):547-57. doi: 10.1046/j.1365-2443.1997.1440342.x. Genes Cells. 1997. PMID: 9413996 - Translational control in production of transposase and in transposition of insertion sequence IS3.
Sekine Y, Eisaki N, Ohtsubo E. Sekine Y, et al. J Mol Biol. 1994 Feb 4;235(5):1406-20. doi: 10.1006/jmbi.1994.1097. J Mol Biol. 1994. PMID: 8107082 - Copy-out-Paste-in Transposition of IS911: A Major Transposition Pathway.
Chandler M, Fayet O, Rousseau P, Ton Hoang B, Duval-Valentin G. Chandler M, et al. Microbiol Spectr. 2015 Aug;3(4). doi: 10.1128/microbiolspec.MDNA3-0031-2014. Microbiol Spectr. 2015. PMID: 26350305 Review. - IS1-encoded proteins, InsA and the InsA-B'-InsB transframe protein (transposase): functions deduced from their DNA-binding ability.
Sekino N, Sekine Y, Ohtsubo E. Sekino N, et al. Adv Biophys. 1995;31:209-22. doi: 10.1016/0065-227x(95)99393-4. Adv Biophys. 1995. PMID: 7625275 Review.
Cited by
- IS911 transpososome assembly as analysed by tethered particle motion.
Pouget N, Turlan C, Destainville N, Salomé L, Chandler M. Pouget N, et al. Nucleic Acids Res. 2006;34(16):4313-23. doi: 10.1093/nar/gkl420. Epub 2006 Aug 21. Nucleic Acids Res. 2006. PMID: 16923775 Free PMC article. - The diversity of prokaryotic DDE transposases of the mutator superfamily, insertion specificity, and association with conjugation machineries.
Guérillot R, Siguier P, Gourbeyre E, Chandler M, Glaser P. Guérillot R, et al. Genome Biol Evol. 2014 Feb;6(2):260-72. doi: 10.1093/gbe/evu010. Genome Biol Evol. 2014. PMID: 24418649 Free PMC article. - Terminal inverted repeats of insertion sequence IS30 serve as targets for transposition.
Olasz F, Farkas T, Kiss J, Arini A, Arber W. Olasz F, et al. J Bacteriol. 1997 Dec;179(23):7551-8. doi: 10.1128/jb.179.23.7551-7558.1997. J Bacteriol. 1997. PMID: 9393723 Free PMC article. - One-ended insertion of IS911.
Polard P, Seroude L, Fayet O, Prère MF, Chandler M. Polard P, et al. J Bacteriol. 1994 Feb;176(4):1192-6. doi: 10.1128/jb.176.4.1192-1196.1994. J Bacteriol. 1994. PMID: 8106332 Free PMC article. - ISD1, an insertion element from the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough: structure, transposition, and distribution.
Fu R, Voordouw G. Fu R, et al. Appl Environ Microbiol. 1998 Jan;64(1):53-61. doi: 10.1128/AEM.64.1.53-61.1998. Appl Environ Microbiol. 1998. PMID: 9435062 Free PMC article.
References
- Gene. 1987;52(2-3):147-54 - PubMed
- J Mol Biol. 1980 Apr;138(2):179-207 - PubMed
- Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92 - PubMed
- Cold Spring Harb Symp Quant Biol. 1984;49:251-60 - PubMed
- Gene. 1984 Sep;29(3):303-13 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources