An embryonically expressed gene is a target for c-Myc regulation via the c-Myc-binding sequence - PubMed (original) (raw)

. 1992 Dec;6(12B):2513-23.

doi: 10.1101/gad.6.12b.2513.

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An embryonically expressed gene is a target for c-Myc regulation via the c-Myc-binding sequence

N Benvenisty et al. Genes Dev. 1992 Dec.

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Abstract

We have used a subtraction/coexpression strategy involving two different tumors derived from c-myc-bearing transgenic mice to identify a gene that is a target for c-Myc regulation. The gene, expressed in certain embryonic and adult tissues and in several (but not all) c-myc-based tumors, bears a functional c-Myc-binding sequence located 3' to its transcription start site. This sequence is required for the binding of a nuclear protein complex which, by antibody analysis, includes c-Myc. This site is also required for expression of a reporter gene in chimeric constructs transfected into c-myc-overexpressing cells and, conversely, requires c-myc cotransfection for its enhanced expression in COS cells. Furthermore, transfection of c-myc blocks the normal down-regulation of this gene, which occurs in embryonic stem cells as they undergo differentiation. This target gene encodes an anonymous cDNA (ECA39) found previously to be amplified in a teratocarcinoma cell line.

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