Identification, genetic analysis and characterization of a sugar-non-specific nuclease from the cyanobacterium Anabaena sp. PCC 7120 - PubMed (original) (raw)

Comparative Study

Identification, genetic analysis and characterization of a sugar-non-specific nuclease from the cyanobacterium Anabaena sp. PCC 7120

A M Muro-Pastor et al. Mol Microbiol. 1992 Oct.

Abstract

A nuclease that could be recovered from the supernatant of cultures, as well as from cell-free extracts, of the cyanobacterium Anabaena sp. PCC 7120 was identified as a 29 kDa polypeptide by its ability to degrade DNA after electrophoresis in DNA-containing SDS-polyacrylamide gels. Some clones of a gene library of strain PCC 7120 established in Escherichia coli were found to produce the 29 kDa nuclease. The nucA gene encoding this nuclease was subcloned and sequenced. The deduced polypeptide, NucA, had a molecular weight of 29,650, presented a presumptive signal peptide in its N-terminal region and showed homology to the products of the nuc gene from Serratia marcescens and the NUC1 gene from Saccharomyces cerevisiae. The NucA protein from Anabaena itself, or from the cloned nucA gene expressed in E. coli, catalysed the degradation of both RNA and DNA, had the potential to act as an endonuclease, and functioned best in the presence of Mn2+ or Mg2+. An Anabaena nucA insertional mutant was generated which failed to produce the 29 kDa nuclease.

PubMed Disclaimer

Cited by

Cloning and characterization of a novel nuclease from shrimp hepatopancreas, and prediction of its active site.
Wang WY, Liaw SH, Liao TH. Wang WY, et al. Biochem J. 2000 Mar 15;346 Pt 3(Pt 3):799-804. Biochem J. 2000. PMID: 10698709 Free PMC article.
Identification of a new family of putative PD-(D/E)XK nucleases with unusual phylogenomic distribution and a new type of the active site.
Feder M, Bujnicki JM. Feder M, et al. BMC Genomics. 2005 Feb 18;6:21. doi: 10.1186/1471-2164-6-21. BMC Genomics. 2005. PMID: 15720711 Free PMC article.
Characterization of a restriction barrier and electrotransformation of the cyanobacterium Nostoc PCC 7121.
Moser DP, Zarka D, Kallas T. Moser DP, et al. Arch Microbiol. 1993;160(3):229-37. doi: 10.1007/BF00249129. Arch Microbiol. 1993. PMID: 8215799
Identification of catalytically relevant amino acids of the extracellular Serratia marcescens endonuclease by alignment-guided mutagenesis.
Friedhoff P, Gimadutdinow O, Pingoud A. Friedhoff P, et al. Nucleic Acids Res. 1994 Aug 25;22(16):3280-7. doi: 10.1093/nar/22.16.3280. Nucleic Acids Res. 1994. PMID: 8078761 Free PMC article.
Cloning, sequencing, and characterization of the nucH gene encoding an extracellular nuclease from Aeromonas hydrophila JMP636.
Dodd HN, Pemberton JM. Dodd HN, et al. J Bacteriol. 1996 Jul;178(13):3926-33. doi: 10.1128/jb.178.13.3926-3933.1996. J Bacteriol. 1996. PMID: 8682799 Free PMC article.

Identification, genetic analysis and characterization of a sugar-non-specific nuclease from the cyanobacterium Anabaena sp. PCC 7120 - PubMed (original) (raw)