Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction - PubMed (original) (raw)

. 1992 Aug 14;257(5072):967-71.

doi: 10.1126/science.1354393.

Affiliations

• PMID: 1354393
• DOI: 10.1126/science.1354393

Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction

P Liang et al. Science. 1992.

Abstract

Effective methods are needed to identify and isolate those genes that are differentially expressed in various cells or under altered conditions. This report describes a method to separate and clone individual messenger RNAs (mRNAs) by means of the polymerase chain reaction. The key element is to use a set of oligonucleotide primers, one being anchored to the polyadenylate tail of a subset of mRNAs, the other being short and arbitrary in sequence so that it anneals at different positions relative to the first primer. The mRNA subpopulations defined by these primer pairs were amplified after reverse transcription and resolved on a DNA sequencing gel. When multiple primer sets were used, reproducible patterns of amplified complementary DNA fragments were obtained that showed strong dependence on sequence specificity of either primer.

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Comment in

• Alternatives to 35S as a label for the differential display of eukaryotic messenger RNA.
  Trentmann SM, van der Knaap E, Kende H. Trentmann SM, et al. Science. 1995 Feb 24;267(5201):1186-7. doi: 10.1126/science.7855603. Science. 1995. PMID: 7855603 No abstract available.
• Expression profiling: opportunities and pitfalls and impact on the study and management of allergic diseases.
  Ono SJ, Nakamura T, Ohbayashi M, Dawson M, Ikeda Y, Nugent AK, Toda M, Jay G. Ono SJ, et al. J Allergy Clin Immunol. 2003 Dec;112(6):1050-6. doi: 10.1016/j.jaci.2003.09.022. J Allergy Clin Immunol. 2003. PMID: 14657857 Review. No abstract available.

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