Comparison of molecular typing methods for Candida albicans - PubMed (original) (raw)

Comparative Study

Comparison of molecular typing methods for Candida albicans

P T Magee et al. J Clin Microbiol. 1992 Oct.

Abstract

Four molecular approaches to determining the types of Candida albicans strains were compared. The strains used were those whose repeated DNA (ribosomal and mitochondrial) EcoRI restriction fragment length polymorphisms (RFLP) were determined by Stevens et al. (D. A. Stevens, F. C. Odds, and S. Scherer, Rev. Infect. Dis. 12:258-266, 1990). Scherer and Stevens (S. Scherer and D. A. Stevens, Proc. Natl. Acad. Sci. USA 85:1452-1456, 1988) used the same strains to examine the Southern blots of genomic EcoRI digests probed with the repeated sequence 27A. The results of these investigators were compared with determinations of RFLPs generated from repeated DNA by the enzyme HinfI and examination of the karyotypes of strains under two sets of conditions, one for the smaller chromosomes and one for the larger ones. Analysis of RFLPs of repeated DNA is most convenient but shows the lowest degree of resolution. Use of the repeated sequence and use of karyotype have very high resolution, but the former method is more convenient than the latter. HinfI digestion is more sensitive than EcoRI digestion but equally convenient. By using all four methods, separate types were identified for 18 of the 20 strains examined.

PubMed Disclaimer

References

    1. Rev Infect Dis. 1990 Mar-Apr;12(2):258-66 - PubMed
    1. Nucleic Acids Res. 1987 Apr 24;15(8):3625 - PubMed
    1. J Clin Microbiol. 1991 May;29(5):916-22 - PubMed
    1. J Clin Microbiol. 1989 Apr;27(4):681-90 - PubMed
    1. J Clin Microbiol. 1989 Jun;27(6):1392-4 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources