Drosophila projectin: relatedness to titin and twitchin and correlation with lethal(4) 102 CDa and bent-dominant mutants - PubMed (original) (raw)
Comparative Study
. 1992 Jul 22;249(1324):33-40.
doi: 10.1098/rspb.1992.0080.
Affiliations
- PMID: 1359548
- DOI: 10.1098/rspb.1992.0080
Comparative Study
Drosophila projectin: relatedness to titin and twitchin and correlation with lethal(4) 102 CDa and bent-dominant mutants
C C Fyrberg et al. Proc Biol Sci. 1992.
Abstract
We have investigated projectin, a large protein of insect muscles, in Drosophila melanogaster. The 5.3 kilobases of coding sequence reported here contains Class I and Class II motifs characteristic of titin and twitchin, arranged in a three domain ... [II-I-I] [II-I-I] ... pattern. Two mutants mapped to the location of the projectin gene in the 102C subdivision of chromosome 4, lethal(4) 102 CDa and bent-Dominant, have DNA rearrangements within their projectin gene. The lethal(4) 102 CDa mutant has a 141 nucleotide insertion containing stop codons in all three reading frames within an exon sequence, showing that it cannot synthesize normal projectin. Both bent-Dominant and lethal(4) 102 CDa homozygotes die at the completion of embryogenesis because they are unable to escape the egg vitelline membrane. We propose that this hatching failure is due to muscle weakness caused by projectin defects.
Similar articles
- Drosophila has a twitchin/titin-related gene that appears to encode projectin.
Ayme-Southgate A, Vigoreaux J, Benian G, Pardue ML. Ayme-Southgate A, et al. Proc Natl Acad Sci U S A. 1991 Sep 15;88(18):7973-7. doi: 10.1073/pnas.88.18.7973. Proc Natl Acad Sci U S A. 1991. PMID: 1910171 Free PMC article. - Both synchronous and asynchronous muscle isoforms of projectin (the Drosophila bent locus product) contain functional kinase domains.
Ayme-Southgate A, Southgate R, Saide J, Benian GM, Pardue ML. Ayme-Southgate A, et al. J Cell Biol. 1995 Feb;128(3):393-403. doi: 10.1083/jcb.128.3.393. J Cell Biol. 1995. PMID: 7844153 Free PMC article. - Structure of the Drosophila projectin protein: isoforms and implication for projectin filament assembly.
Daley J, Southgate R, Ayme-Southgate A. Daley J, et al. J Mol Biol. 1998 May 29;279(1):201-10. doi: 10.1006/jmbi.1998.1756. J Mol Biol. 1998. PMID: 9636710 - Titin-related proteins in invertebrate muscles.
Ziegler C. Ziegler C. Comp Biochem Physiol A Physiol. 1994 Dec;109(4):823-33. doi: 10.1016/0300-9629(94)90230-5. Comp Biochem Physiol A Physiol. 1994. PMID: 7828026 Review. - Twitchin and related giant Ig superfamily members of C. elegans and other invertebrates.
Benian GM, Tang X, Tinley TL. Benian GM, et al. Adv Biophys. 1996;33:183-98. Adv Biophys. 1996. PMID: 8922113 Review. No abstract available.
Cited by
- D-Titin: a giant protein with dual roles in chromosomes and muscles.
Machado C, Andrew DJ. Machado C, et al. J Cell Biol. 2000 Oct 30;151(3):639-52. doi: 10.1083/jcb.151.3.639. J Cell Biol. 2000. PMID: 11062264 Free PMC article. - The evolution of titin and related giant muscle proteins.
Higgins DG, Labeit S, Gautel M, Gibson TJ. Higgins DG, et al. J Mol Evol. 1994 Apr;38(4):395-404. doi: 10.1007/BF00163156. J Mol Evol. 1994. PMID: 8007007 - Genomic organization of the autonomous regulatory domain of eyeless locus in Drosophila melanogaster.
Verma S, Pathak RU, Mishra RK. Verma S, et al. G3 (Bethesda). 2021 Dec 8;11(12):jkab338. doi: 10.1093/g3journal/jkab338. G3 (Bethesda). 2021. PMID: 34570231 Free PMC article. - A versatile two-step CRISPR- and RMCE-based strategy for efficient genome engineering in Drosophila.
Zhang X, Koolhaas WH, Schnorrer F. Zhang X, et al. G3 (Bethesda). 2014 Oct 15;4(12):2409-18. doi: 10.1534/g3.114.013979. G3 (Bethesda). 2014. PMID: 25324299 Free PMC article. - Assembly of thick filaments and myofibrils occurs in the absence of the myosin head.
Cripps RM, Suggs JA, Bernstein SI. Cripps RM, et al. EMBO J. 1999 Apr 1;18(7):1793-804. doi: 10.1093/emboj/18.7.1793. EMBO J. 1999. PMID: 10202143 Free PMC article.