Slow diffusion of proteins in the yeast plasma membrane allows polarity to be maintained by endocytic cycling - PubMed (original) (raw)
. 2003 Sep 16;13(18):1636-40.
doi: 10.1016/j.cub.2003.09.001.
Affiliations
- PMID: 13678596
- DOI: 10.1016/j.cub.2003.09.001
Free article
Slow diffusion of proteins in the yeast plasma membrane allows polarity to be maintained by endocytic cycling
Javier Valdez-Taubas et al. Curr Biol. 2003.
Free article
Abstract
Many cells show a polarized distribution of some plasma membrane proteins, which may be maintained either by a diffusion barrier or kinetically: as first demonstrated in fibroblasts, locally exocytosed proteins will remain polarized if they are endocytosed and recycled before they can diffuse to equilibrium. In yeast, actin cables direct exocytosis to the bud and to the tips of polarized mating intermediates termed shmoos. A septin ring at the bud neck retains some proteins, but shmoos lack this. Here, we show that the exocytic SNARE Snc1 is kinetically polarized. It is concentrated at bud and shmoo tips, and this requires its endocytosis. Kinetic polarization is possible in these small cells because proteins diffuse much more slowly in the yeast plasma membrane than would be expected from measurements in animal cells. Slow diffusion requires neither the cell wall nor polymerized actin, but it is affected in the ergosterol synthesis mutant erg6. Other proteins also require endocytosis for efficient polarization, and the plasma membrane SNARE Sso1 can be polarized merely by appending an endocytic signal. Thus, despite their small size, yeast cells can use localized exocytosis and endocytic recycling as a simple mechanism to maintain polarity.
Similar articles
- Plasma membrane polarization during mating in yeast cells.
Proszynski TJ, Klemm R, Bagnat M, Gaus K, Simons K. Proszynski TJ, et al. J Cell Biol. 2006 Jun 19;173(6):861-6. doi: 10.1083/jcb.200602007. Epub 2006 Jun 12. J Cell Biol. 2006. PMID: 16769822 Free PMC article. - Septin-dependent compartmentalization of the endoplasmic reticulum during yeast polarized growth.
Luedeke C, Frei SB, Sbalzarini I, Schwarz H, Spang A, Barral Y. Luedeke C, et al. J Cell Biol. 2005 Jun 20;169(6):897-908. doi: 10.1083/jcb.200412143. J Cell Biol. 2005. PMID: 15967812 Free PMC article. - Yeast endocytic adaptor AP-2 binds the stress sensor Mid2 and functions in polarized cell responses.
Chapa-y-Lazo B, Allwood EG, Smaczynska-de Rooij II, Snape ML, Ayscough KR. Chapa-y-Lazo B, et al. Traffic. 2014 May;15(5):546-57. doi: 10.1111/tra.12155. Epub 2014 Feb 25. Traffic. 2014. PMID: 24460703 Free PMC article. - Polarization of cell growth in yeast.
Pruyne D, Bretscher A. Pruyne D, et al. J Cell Sci. 2000 Feb;113 ( Pt 4):571-85. doi: 10.1242/jcs.113.4.571. J Cell Sci. 2000. PMID: 10652251 Review. - How Diffusion Impacts Cortical Protein Distribution in Yeasts.
Moran KD, Lew DJ. Moran KD, et al. Cells. 2020 Apr 30;9(5):1113. doi: 10.3390/cells9051113. Cells. 2020. PMID: 32365827 Free PMC article. Review.
Cited by
- Evaluation of Lipids and Lipid-Related Transcripts in Human and Ovine Theca Cells and an in Vitro Mouse Model Exposed to the Obesogen Chemical Tributyltin.
Pascuali N, Pu Y, Waye AA, Pearl S, Martin D, Sutton A, Shikanov A, Veiga-Lopez A. Pascuali N, et al. Environ Health Perspect. 2024 Apr;132(4):47009. doi: 10.1289/EHP13955. Epub 2024 Apr 17. Environ Health Perspect. 2024. PMID: 38630605 Free PMC article. - SNARE Protein Snc1 Is Essential for Vesicle Trafficking, Membrane Fusion and Protein Secretion in Fungi.
Adnan M, Islam W, Waheed A, Hussain Q, Shen L, Wang J, Liu G. Adnan M, et al. Cells. 2023 Jun 5;12(11):1547. doi: 10.3390/cells12111547. Cells. 2023. PMID: 37296667 Free PMC article. Review. - Recycling of cell surface membrane proteins from yeast endosomes is regulated by ubiquitinated Ist1.
Laidlaw KME, Calder G, MacDonald C. Laidlaw KME, et al. J Cell Biol. 2022 Nov 7;221(11):e202109137. doi: 10.1083/jcb.202109137. Epub 2022 Sep 20. J Cell Biol. 2022. PMID: 36125415 Free PMC article. - Golgi-Bypass Is a Major Unconventional Route for Translocation to the Plasma Membrane of Non-Apical Membrane Cargoes in Aspergillus nidulans.
Dimou S, Dionysopoulou M, Sagia GM, Diallinas G. Dimou S, et al. Front Cell Dev Biol. 2022 Apr 7;10:852028. doi: 10.3389/fcell.2022.852028. eCollection 2022. Front Cell Dev Biol. 2022. PMID: 35465316 Free PMC article. - Membrane thickness, lipid phase and sterol type are determining factors in the permeability of membranes to small solutes.
Frallicciardi J, Melcr J, Siginou P, Marrink SJ, Poolman B. Frallicciardi J, et al. Nat Commun. 2022 Mar 25;13(1):1605. doi: 10.1038/s41467-022-29272-x. Nat Commun. 2022. PMID: 35338137 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous