Specificity and T cell receptor beta chain usage of a human collagen type II-reactive T cell clone derived from a healthy individual - PubMed (original) (raw)

Specificity and T cell receptor beta chain usage of a human collagen type II-reactive T cell clone derived from a healthy individual

T Yan et al. Eur J Immunol. 1992 Jan.

Abstract

Collagen type II (CII) is a cartilage-specific matrix compound well known as an inducer of an experimental, T cell-dependent autoimmune arthritis, a disease which shows some similarities to human rheumatoid arthritis. Here we report on an HLA-DR7-restricted human CD4 T cell clone (TC9), which was isolated from a healthy donor and recognizes human CII. After screening CNBr fragments of CII and tryptic fragments derived thereof, the T cell epitope could be mapped to amino acid residues 271-285 of the triple helical region of CII that are located within CNBr fragment 11 [alpha 1 (II) CB11]. This epitope was confirmed by a synthetic peptide stimulatory for TC9. The T cell receptor beta chain of TC9 was cloned using the polymerase chain reaction; it comprises V beta 6.7 and contains besides J beta 2.3 and C beta 2 an as yet undescribed sequence for the D segment.

PubMed Disclaimer

Cited by

Predominant selection of T cells specific for the glycosylated collagen type II epitope (263-270) in humanized transgenic mice and in rheumatoid arthritis.
Bäcklund J, Carlsen S, Höger T, Holm B, Fugger L, Kihlberg J, Burkhardt H, Holmdahl R. Bäcklund J, et al. Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9960-5. doi: 10.1073/pnas.132254199. Epub 2002 Jun 27. Proc Natl Acad Sci U S A. 2002. PMID: 12089323 Free PMC article.
Coupling of peripheral tolerance to endogenous interleukin 10 promotes effective modulation of myelin-activated T cells and ameliorates experimental allergic encephalomyelitis.
Legge KL, Min B, Bell JJ, Caprio JC, Li L, Gregg RK, Zaghouani H. Legge KL, et al. J Exp Med. 2000 Jun 19;191(12):2039-52. doi: 10.1084/jem.191.12.2039. J Exp Med. 2000. PMID: 10859329 Free PMC article.
[Pathogenesis of rheumatoid arthritis].
Geiler T, Lorenz HM, Kalden JR, Manger B. Geiler T, et al. Med Klin (Munich). 1997 Jun 15;92(6):347-53. doi: 10.1007/BF03044776. Med Klin (Munich). 1997. PMID: 9297067 Review. German.
Systemic versus cartilage-specific expression of a type II collagen-specific T-cell epitope determines the level of tolerance and susceptibility to arthritis.
Malmström V, Michaëlsson E, Burkhardt H, Mattsson R, Vuorio E, Holmdahl R. Malmström V, et al. Proc Natl Acad Sci U S A. 1996 Apr 30;93(9):4480-5. doi: 10.1073/pnas.93.9.4480. Proc Natl Acad Sci U S A. 1996. PMID: 8633094 Free PMC article.
Molecular and cellular mechanisms of joint destruction in rheumatoid arthritis: two cellular mechanisms explain joint destruction?
Gay S, Gay RE, Koopman WJ. Gay S, et al. Ann Rheum Dis. 1993 Mar;52 Suppl 1(Suppl 1):S39-47. doi: 10.1136/ard.52.suppl_1.s39. Ann Rheum Dis. 1993. PMID: 8481058 Free PMC article. Review. No abstract available.

Specificity and T cell receptor beta chain usage of a human collagen type II-reactive T cell clone derived from a healthy individual - PubMed (original) (raw)