Cytokine stimulation of multilineage hematopoiesis from immature human cells engrafted in SCID mice - PubMed (original) (raw)
. 1992 Feb 28;255(5048):1137-41.
doi: 10.1126/science.1372131.
Affiliations
- PMID: 1372131
- DOI: 10.1126/science.1372131
Cytokine stimulation of multilineage hematopoiesis from immature human cells engrafted in SCID mice
T Lapidot et al. Science. 1992.
Abstract
Severe combined immunodeficient (SCID) mice transplanted with human bone marrow were treated with human mast cell growth factor, a fusion of interleukin-3 and granulocyte-macrophage colony-stimulating factor (PIXY321), or both, starting immediately or 1 month later. Immature human cells repopulated the mouse bone marrow with differentiated human cells of multiple myeloid and lymphoid lineages; inclusion of erythropoietin resulted in human red cells in the peripheral blood. The bone marrow of growth factor-treated mice contained both multipotential and committed myeloid and erythroid progenitors, whereas mice not given growth factors had few human cells and only granulocyte-macrophage progenitors. Thus, this system allows the detection of immature human cells, identification of the growth factors that regulate them, and the establishment of animal models of human hematopoietic diseases.
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