Rapid visual assay of cytotoxic T-cell specificity utilizing synthetic peptide induced T-cell-T-cell killing - PubMed (original) (raw)
Rapid visual assay of cytotoxic T-cell specificity utilizing synthetic peptide induced T-cell-T-cell killing
S R Burrows et al. Immunology. 1992 May.
Abstract
Synthetic peptides are widely used to define the specificity of CD8+ cytotoxic T-lymphocyte (CTL) clones. When many peptides need to be tested by the standard chromium release assay large numbers of a CTL clone are required. Specific synthetic peptide epitopes induce CTL clones to kill each other. This phenomenon can be directly visualized using an inverted microscope and forms the basis for a convenient assay, which can be performed with as few as 100 CTL per peptide and does not require radiolabelled targets.
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References
- Immunology. 1990 Jun;70(2):223-9 - PubMed
- Eur J Immunol. 1992 Jan;22(1):191-5 - PubMed
- J Exp Med. 1990 Jun 1;171(6):2011-24 - PubMed
- Proc Natl Acad Sci U S A. 1990 Nov;87(22):9015-9 - PubMed
- J Virol. 1990 Aug;64(8):3974-6 - PubMed
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