Identification of a specific glycoprotein ligand for P-selectin (CD62) on myeloid cells - PubMed (original) (raw)
Identification of a specific glycoprotein ligand for P-selectin (CD62) on myeloid cells
K L Moore et al. J Cell Biol. 1992 Jul.
Abstract
P-selectin (CD62, GMP-140, PADGEM), a Ca(2+)-dependent lectin on activated platelets and endothelium, functions as a receptor for myeloid cells by interacting with sialylated, fucosylated lactosaminoglycans. P-selectin binds to a limited number of protease-sensitive sites on myeloid cells, but the protein(s) that carry the glycans recognized by P-selectin are unknown. Blotting of neutrophil or HL-60 cell membrane extracts with [125I]P-selectin and affinity chromatography of [3H]glucosamine-labeled HL-60 cell extracts were used to identify P-selectin ligands. A major ligand was identified with an approximately 250,000 M(r) under nonreducing conditions and approximately 120,000 under reducing conditions. Binding of P-selectin to the ligand was Ca2+ dependent and was blocked by mAbs to P-selectin. Brief sialidase digestion of the ligand increased its apparent molecular weight; however, prolonged digestion abolished binding of P-selectin. Peptide:N-glycosidase F treatment reduced the apparent molecular weight of the ligand by approximately 3,000 but did not affect P-selectin binding. Western blot and immunodepletion experiments indicated that the ligand was not lamp-1, lamp-2, or L-selectin, which carry sialyl Le(x), nor was it leukosialin, a heavily sialylated glycoprotein of similar molecular weight. The preferential interaction of the ligand with P-selectin suggests that it may play a role in adhesion of myeloid cells to activated platelets and endothelial cells.
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References
- J Biol Chem. 1983 Dec 25;258(24):15261-73 - PubMed
- J Cell Biol. 1990 Dec;111(6 Pt 1):2757-64 - PubMed
- J Biol Chem. 1984 Sep 10;259(17):10925-35 - PubMed
- J Biol Chem. 1983 Oct 25;258(20):12465-71 - PubMed
- J Biol Chem. 1984 Aug 10;259(15):9799-804 - PubMed
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