Molecular epidemiology of Pseudomonas cepacia determined by polymerase chain reaction ribotyping - PubMed (original) (raw)

Molecular epidemiology of Pseudomonas cepacia determined by polymerase chain reaction ribotyping

J R Kostman et al. J Clin Microbiol. 1992 Aug.

Abstract

Traditional ribotyping detects genomic restriction fragment length polymorphisms by probing chromosomal DNA with rRNA. Although it is a powerful method for determining the molecular epidemiology of bacterial pathogens, technical difficulties limit its application. As an alternative, polymorphisms were sought in the 16S-23S spacer regions of bacterial rRNA genes by use of the polymerase chain reaction (PCR). Chromosomal DNA from isolates of Pseudomonas cepacia was used as a template in the PCR with oligonucleotide primers complementary to highly conserved sequences flanking the spacer regions of the rRNA genes. Length polymorphisms in the amplified DNA distinguished unrelated isolates of P. cepacia. Isolates of P. cepacia previously implicated in person-to-person transmission were shown to have identical amplification patterns. These data demonstrate the utility of this new PCR ribotyping method for determining the molecular epidemiology of bacterial species.

PubMed Disclaimer

References

    1. Lancet. 1990 Nov 3;336(8723):1094-6 - PubMed
    1. Infect Immun. 1980 Dec;30(3):709-17 - PubMed
    1. Res Microbiol. 1990 Feb;141(2):159-71 - PubMed
    1. Curr Genet. 1990 Oct;18(3):203-5 - PubMed
    1. Science. 1988 Jan 29;239(4839):487-91 - PubMed

MeSH terms

Substances

LinkOut - more resources