Predominant naturally processed peptides bound to HLA-DR1 are derived from MHC-related molecules and are heterogeneous in size - PubMed (original) (raw)
Comparative Study
. 1992 Aug 27;358(6389):764-8.
doi: 10.1038/358764a0.
Affiliations
- PMID: 1380674
- DOI: 10.1038/358764a0
Comparative Study
Predominant naturally processed peptides bound to HLA-DR1 are derived from MHC-related molecules and are heterogeneous in size
R M Chicz et al. Nature. 1992.
Abstract
Peptides bound to class I molecules are 8-10 amino acids long, and possess a binding motif representative of peptides that bind to a given class I allele. In the only published study of naturally processed peptides bound to class II molecules (mouse I-Ab and I-Eb), these peptides were longer (13-17 amino acids) and had heterogenous carboxy terminals but precise amino-terminal truncations. Here we report the characterization of acid-eluted peptides bound to HLA-DR1 by high-performance liquid chromatography, mass spectrometry and microsequencing analyses. The relative molecular masses of the peptides varied between 1,602 and 2,996 (13-25 residues), the most abundant individual M(r) values being between 1,700 and 1,800, corresponding to an average peptide length of 15 residues. Complete sequence data were obtained for twenty peptides derived from five epitopes, of which all but one were from self proteins. These peptides represented sets nested at both the N- and C-terminal ends. Binding experiments confirmed that all of the isolated peptides had high affinity for the groove of DR1. Alignment of the peptides bound to HLA-DR1 and the sequences of 35 known HLA-DR1-binding peptides revealed a putative motif. Although peptides bound to class II molecules may have some related features (due to the nonpolymorphic HLA-DR alpha-chain), accounting for degenerate binding to different alleles, particular amino acids in the HLA-DR beta-chains presumably define allelic specificity of peptide binding.
Similar articles
- A recombinant single-chain human class II MHC molecule (HLA-DR1) as a covalently linked heterotrimer of alpha chain, beta chain, and antigenic peptide, with immunogenicity in vitro and reduced affinity for bacterial superantigens.
Zhu X, Bavari S, Ulrich R, Sadegh-Nasseri S, Ferrone S, McHugh L, Mage M. Zhu X, et al. Eur J Immunol. 1997 Aug;27(8):1933-41. doi: 10.1002/eji.1830270817. Eur J Immunol. 1997. PMID: 9295029 - Studies on activities of invariant chain peptides on releasing or exchanging of antigenic peptides at human leukocyte antigen-DR1.
Xu M, Jackson R, Adams S, Humphreys RE. Xu M, et al. Arzneimittelforschung. 1999 Sep;49(9):791-9. Arzneimittelforschung. 1999. PMID: 10514908 - Interaction of MHC class II molecules with the invariant chain: role of the invariant chain (81-90) region.
Stumptner P, Benaroch P. Stumptner P, et al. EMBO J. 1997 Oct 1;16(19):5807-18. doi: 10.1038/sj.emboj.7590555. EMBO J. 1997. PMID: 9312039 Free PMC article. - Class I MHC-peptide interaction: structural and functional aspects.
Ruppert J, Kubo RT, Sidney J, Grey HM, Sette A. Ruppert J, et al. Behring Inst Mitt. 1994 Jul;(94):48-60. Behring Inst Mitt. 1994. PMID: 7998914 Review. - [Peptide binding motif for HLA class I and autoimmune disease].
Sakaguchi T, Takiguchi M. Sakaguchi T, et al. Nihon Rinsho. 1997 Jun;55(6):1397-402. Nihon Rinsho. 1997. PMID: 9200923 Review. Japanese.
Cited by
- Advancing one health vaccination: In silico design and evaluation of a multi-epitope subunit vaccine against Nipah virus for cross-species immunization using immunoinformatics and molecular modeling.
Banico EC, Sira EMJS, Fajardo LE, Dulay ANG, Odchimar NMO, Simbulan AM, Orosco FL. Banico EC, et al. PLoS One. 2024 Sep 26;19(9):e0310703. doi: 10.1371/journal.pone.0310703. eCollection 2024. PLoS One. 2024. PMID: 39325755 Free PMC article. - Harnessing microbial antigens as cancer antigens: a promising avenue for cancer immunotherapy.
Zhang T, Zhang X, Chen J, Zhang X, Zhang Y. Zhang T, et al. Front Immunol. 2024 Jul 30;15:1411490. doi: 10.3389/fimmu.2024.1411490. eCollection 2024. Front Immunol. 2024. PMID: 39139570 Free PMC article. Review. - FASTMAP-a flexible and scalable immunopeptidomics pipeline for HLA- and antigen-specific T-cell epitope mapping based on artificial antigen-presenting cells.
Weisbrod L, Capriotti L, Hofmann M, Spieler V, Dersch H, Voedisch B, Schmidt P, Knake S. Weisbrod L, et al. Front Immunol. 2024 May 8;15:1386160. doi: 10.3389/fimmu.2024.1386160. eCollection 2024. Front Immunol. 2024. PMID: 38779658 Free PMC article. - Origins, Technological Advancement, and Applications of Peptidomics.
Schrader M. Schrader M. Methods Mol Biol. 2024;2758:3-47. doi: 10.1007/978-1-0716-3646-6_1. Methods Mol Biol. 2024. PMID: 38549006 - Neoself Antigens Presented on MHC Class II Molecules in Autoimmune Diseases.
Jin H, Arase H. Jin H, et al. Adv Exp Med Biol. 2024;1444:51-65. doi: 10.1007/978-981-99-9781-7_4. Adv Exp Med Biol. 2024. PMID: 38467972
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials