Identification of ezrin as an 81-kDa tyrosine-phosphorylated protein in T cells - PubMed (original) (raw)
. 1992 Sep 15;149(6):1847-52.
Affiliations
- PMID: 1381389
Identification of ezrin as an 81-kDa tyrosine-phosphorylated protein in T cells
M Egerton et al. J Immunol. 1992.
Abstract
We have used APT affinity purification to isolate tyrosine-phosphorylated proteins from MRL lpr/lpr (lpr) mouse T cells. One such protein is pp81 ezrin, previously identified as a tyrosine-phosphorylated protein in epidermal growth factor-stimulated A431 carcinoma cells. Biochemical analyses in A431 and gastric parietal cells have revealed ezrin to be a cytoskeleton-associated cytosolic protein. In Jurkat T cells, however, using similar methods we have shown ezrin to be a cytosolic protein with no measurable cytoskeletal association. We also observed no increases in ezrin tyrosine phosphorylation in TCR-stimulated Jurkat T cells, unless the cells were pretreated with protein tyrosine phosphatase inhibitors, suggesting that T cell ezrin tyrosine phosphorylation is tightly controlled by protein tyrosine phosphatases. The fraction of tyrosine phosphorylated ezrin in lpr T cells was 5 to 10 times that observed in Jurkat T cells, which along with constitutive TCR-zeta phosphorylation and pp60fyn overexpression, is a feature of the lpr defect.
Similar articles
- Increases in tyrosine phosphorylation are detectable before phospholipase C activation after T cell receptor stimulation.
June CH, Fletcher MC, Ledbetter JA, Samelson LE. June CH, et al. J Immunol. 1990 Mar 1;144(5):1591-9. J Immunol. 1990. PMID: 1689750 - Constitutive tyrosine phosphorylation of the vav proto-oncogene product in MRL/Mp-lpr/lpr mice.
Mimura T, Minota S, Nojima Y, Morino N, Hamasaki K, Furuya H, Yazaki Y. Mimura T, et al. J Immunol. 1997 Mar 15;158(6):2977-83. J Immunol. 1997. PMID: 9058837 - Tyrosine phosphorylation of alpha tubulin in human T lymphocytes.
Ley SC, Verbi W, Pappin DJ, Druker B, Davies AA, Crumpton MJ. Ley SC, et al. Eur J Immunol. 1994 Jan;24(1):99-106. doi: 10.1002/eji.1830240116. Eur J Immunol. 1994. PMID: 7517366 - Multiple signal transduction pathways activated through the T cell receptor for antigen.
Siegel JN, Egerton M, Phillips AF, Samelson LE. Siegel JN, et al. Semin Immunol. 1991 Sep;3(5):325-34. Semin Immunol. 1991. PMID: 1724737 Review.
Cited by
- Inhibition of Ezrin suppresses cell migration and invasion in human nasopharyngeal carcinoma.
Tang Y, Sun X, Yu S, Bie X, Wang J, Ren L. Tang Y, et al. Oncol Lett. 2019 Jul;18(1):553-560. doi: 10.3892/ol.2019.10370. Epub 2019 May 17. Oncol Lett. 2019. PMID: 31289527 Free PMC article. - Ezrin has a COOH-terminal actin-binding site that is conserved in the ezrin protein family.
Turunen O, Wahlström T, Vaheri A. Turunen O, et al. J Cell Biol. 1994 Sep;126(6):1445-53. doi: 10.1083/jcb.126.6.1445. J Cell Biol. 1994. PMID: 8089177 Free PMC article. - Ezrin and moesin function together to promote T cell activation.
Shaffer MH, Dupree RS, Zhu P, Saotome I, Schmidt RF, McClatchey AI, Freedman BD, Burkhardt JK. Shaffer MH, et al. J Immunol. 2009 Jan 15;182(2):1021-32. doi: 10.4049/jimmunol.182.2.1021. J Immunol. 2009. PMID: 19124745 Free PMC article. - The molecular chaperone calnexin is expressed on the surface of immature thymocytes in association with clonotype-independent CD3 complexes.
Wiest DL, Burgess WH, McKean D, Kearse KP, Singer A. Wiest DL, et al. EMBO J. 1995 Jul 17;14(14):3425-33. doi: 10.1002/j.1460-2075.1995.tb07348.x. EMBO J. 1995. PMID: 7628443 Free PMC article. - RAFTK, a novel member of the focal adhesion kinase family, is phosphorylated and associates with signaling molecules upon activation of mature T lymphocytes.
Ganju RK, Hatch WC, Avraham H, Ona MA, Druker B, Avraham S, Groopman JE. Ganju RK, et al. J Exp Med. 1997 Mar 17;185(6):1055-63. doi: 10.1084/jem.185.6.1055. J Exp Med. 1997. PMID: 9091579 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Molecular Biology Databases