Macrophages, T cell receptor usage, and endothelial cell activation in the pancreas at the onset of insulin-dependent diabetes mellitus - PubMed (original) (raw)

Case Reports

Macrophages, T cell receptor usage, and endothelial cell activation in the pancreas at the onset of insulin-dependent diabetes mellitus

A Hänninen et al. J Clin Invest. 1992 Nov.

Abstract

Current knowledge of the phenotype of mononuclear cells accumulating in pancreatic islets in insulin-dependent diabetes (IDDM) and factors determining their homing into the pancreas is limited. Therefore, a pancreas obtained at the onset of IDDM was studied in detail. Cryostat sections were stained for mononuclear cell types, T cell receptor subtypes, and adhesion molecules of vascular endothelium and studied by immunofluorescence microscopy, and peripheral blood mononuclear cells were phenotyped using flow cytometry. Monocytes/macrophages (lysozyme- or CD 14-reactive cells) were identified among other mononuclear cell types in islet infiltrates. V beta 8-positive T cells were overrepresented, but T cells with other V beta s studied (V beta 5, V beta 5.1, V beta 6, V beta 12) were also found. The vascular endothelium of the islets and many small vessels nearby islets strongly expressed intercellular adhesion molecule-1, whereas vascular cell adhesion molecule-1 and E-selectin were totally absent. We conclude: (a) that increased expression of intercellular adhesion molecule-1 on vascular endothelium may increase endothelial adhesion of mononuclear cells and enhance their accumulation in the pancreas during diabetic insulitis; (b) that T cells with certain T cell receptors can be enriched in infiltrated pancreatic islets; and (c) that macrophages and antigen-specific CD 8-positive T cells are involved in pancreatic beta cell destruction at the onset of IDDM.

PubMed Disclaimer

Cited by

Diabetes as a Pancreatic Microvascular Disease-A Pericytic Perspective.
Mateus Gonçalves L, Andrade Barboza C, Almaça J. Mateus Gonçalves L, et al. J Histochem Cytochem. 2024 Mar;72(3):131-148. doi: 10.1369/00221554241236535. Epub 2024 Mar 7. J Histochem Cytochem. 2024. PMID: 38454609 Review.
4-Octyl itaconate attenuates glycemic deterioration by regulating macrophage polarization in mouse models of type 1 diabetes.
He S, Zhao Y, Wang G, Ke Q, Wu N, Lu L, Wu J, Sun S, Jin W, Zhang W, Zhou J. He S, et al. Mol Med. 2023 Mar 14;29(1):31. doi: 10.1186/s10020-023-00626-5. Mol Med. 2023. PMID: 36918798 Free PMC article.
Exploring Computational Data Amplification and Imputation for the Discovery of Type 1 Diabetes (T1D) Biomarkers from Limited Human Datasets.
Alcazar O, Ogihara M, Ren G, Buchwald P, Abdulreda MH. Alcazar O, et al. Biomolecules. 2022 Oct 9;12(10):1444. doi: 10.3390/biom12101444. Biomolecules. 2022. PMID: 36291653 Free PMC article.
Upregulation of HLA class II in pancreatic beta cells from organ donors with type 1 diabetes.
Quesada-Masachs E, Zilberman S, Rajendran S, Chu T, McArdle S, Kiosses WB, Lee JM, Yesildag B, Benkahla MA, Pawlowska A, Graef M, Pfeiffer S, Mikulski Z, von Herrath M. Quesada-Masachs E, et al. Diabetologia. 2022 Feb;65(2):387-401. doi: 10.1007/s00125-021-05619-9. Epub 2021 Dec 21. Diabetologia. 2022. PMID: 34932134
Tolerogenic Dendritic Cell-Based Approaches in Autoimmunity.
Passeri L, Marta F, Bassi V, Gregori S. Passeri L, et al. Int J Mol Sci. 2021 Aug 5;22(16):8415. doi: 10.3390/ijms22168415. Int J Mol Sci. 2021. PMID: 34445143 Free PMC article. Review.

References

1. J Immunol. 1989 Apr 1;142(7):2257-63 - PubMed
1. J Exp Med. 1988 Feb 1;167(2):502-13 - PubMed
1. Nature. 1989 Apr 6;338(6215):512-4 - PubMed
1. Diabetes. 1988 Jul;37(7):989-91 - PubMed
1. Diabetologia. 1988 Jun;31(6):400-2 - PubMed

Macrophages, T cell receptor usage, and endothelial cell activation in the pancreas at the onset of insulin-dependent diabetes mellitus - PubMed (original) (raw)