Suppressor analysis suggests a multistep, cyclic mechanism for protein secretion in Escherichia coli - PubMed (original) (raw)

Suppressor analysis suggests a multistep, cyclic mechanism for protein secretion in Escherichia coli

K Bieker-Brady et al. EMBO J. 1992 Sep.

Abstract

The sec/prl gene products catalyze the translocation of precursor proteins from the cytoplasm of Escherichia coli. Recessive, conditionally lethal mutant alleles of these genes (sec mutations) cause a generalized defect in protein secretion; dominant suppressor mutant alleles (prl mutations) restore export of precursor proteins with altered signal sequences. In prl strains, a precursor protein with a defective signal sequence can be selectively targeted to the suppressor gene product. When a precursor LacZ hybrid protein is used, the targeted prl protein is inactivated by the large, toxic hybrid molecule, a result termed suppressor-directed inactivation (SDI). Using SDI, two different secretion-related complexes can be generated: a pretranslocation complex that contains a hybrid protein with an unprocessed signal sequence, and a translocation complex in which the hybrid protein is jammed in transmembrane orientation with the signal sequence cleaved. Additional Sec proteins that are contained within, and thus sequestered by, each of these complexes can be identified when their functional levels are lowered using the conditional lethal sec mutations. Results of this genetic analysis suggest a multistep pathway for protein secretion in which the translocation machinery assembles on demand.

PubMed Disclaimer

Similar articles

• PrlA (SecY) and PrlG (SecE) interact directly and function sequentially during protein translocation in E. coli.
  Bieker KL, Silhavy TJ. Bieker KL, et al. Cell. 1990 Jun 1;61(5):833-42. doi: 10.1016/0092-8674(90)90193-i. Cell. 1990. PMID: 2111734
• Suppression of signal sequence defects and azide resistance in Escherichia coli commonly result from the same mutations in secA.
  Huie JL, Silhavy TJ. Huie JL, et al. J Bacteriol. 1995 Jun;177(12):3518-26. doi: 10.1128/jb.177.12.3518-3526.1995. J Bacteriol. 1995. PMID: 7768862 Free PMC article.
• secG and temperature modulate expression of azide-resistant and signal sequence suppressor phenotypes of Escherichia coli secA mutants.
  Ramamurthy V, Dapíc V, Oliver D. Ramamurthy V, et al. J Bacteriol. 1998 Dec;180(23):6419-23. doi: 10.1128/JB.180.23.6419-6423.1998. J Bacteriol. 1998. PMID: 9829959 Free PMC article.
• [Dynamic structure change of protein secretion machinery].
  Nishiyama K, Tokuda H. Nishiyama K, et al. Tanpakushitsu Kakusan Koso. 1997 Jan;42(1):1-11. Tanpakushitsu Kakusan Koso. 1997. PMID: 8990942 Review. Japanese. No abstract available.
• SecA protein: autoregulated ATPase catalysing preprotein insertion and translocation across the Escherichia coli inner membrane.
  Oliver DB. Oliver DB. Mol Microbiol. 1993 Jan;7(2):159-65. doi: 10.1111/j.1365-2958.1993.tb01107.x. Mol Microbiol. 1993. PMID: 8446024 Review.

Cited by

• Mycobacterium tuberculosis SatS is a chaperone for the SecA2 protein export pathway.
  Miller BK, Hughes R, Ligon LS, Rigel NW, Malik S, Anjuwon-Foster BR, Sacchettini JC, Braunstein M. Miller BK, et al. Elife. 2019 Jan 3;8:e40063. doi: 10.7554/eLife.40063. Elife. 2019. PMID: 30604681 Free PMC article.
• ATPase active-site electrostatic interactions control the global conformation of the 100 kDa SecA translocase.
  Kim DM, Zheng H, Huang YJ, Montelione GT, Hunt JF. Kim DM, et al. J Am Chem Soc. 2013 Feb 27;135(8):2999-3010. doi: 10.1021/ja306361q. Epub 2013 Feb 14. J Am Chem Soc. 2013. PMID: 23167435 Free PMC article.
• SecA, a remarkable nanomachine.
  Kusters I, Driessen AJ. Kusters I, et al. Cell Mol Life Sci. 2011 Jun;68(12):2053-66. doi: 10.1007/s00018-011-0681-y. Epub 2011 Apr 10. Cell Mol Life Sci. 2011. PMID: 21479870 Free PMC article. Review.
• Mapping an interface of SecY (PrlA) and SecE (PrlG) by using synthetic phenotypes and in vivo cross-linking.
  Harris CR, Silhavy TJ. Harris CR, et al. J Bacteriol. 1999 Jun;181(11):3438-44. doi: 10.1128/JB.181.11.3438-3444.1999. J Bacteriol. 1999. PMID: 10348856 Free PMC article.
• SecY and SecA interact to allow SecA insertion and protein translocation across the Escherichia coli plasma membrane.
  Matsumoto G, Yoshihisa T, Ito K. Matsumoto G, et al. EMBO J. 1997 Nov 3;16(21):6384-93. doi: 10.1093/emboj/16.21.6384. EMBO J. 1997. PMID: 9351821 Free PMC article.

References

1. 1. EMBO J. 1991 Jul;10(7):1749-57 - PubMed
2. 1. Cell. 1982 Aug;30(1):311-9 - PubMed
3. 1. J Bioenerg Biomembr. 1990 Jun;22(3):311-36 - PubMed
4. 1. FEBS Lett. 1990 Aug 20;269(1):96-100 - PubMed
5. 1. J Bioenerg Biomembr. 1990 Jun;22(3):337-51 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • Nature Publishing Group
  • PubMed Central
  • Wiley