UV absorption complicates PCR decontamination - PubMed (original) (raw)
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- PMID: 1389153
UV absorption complicates PCR decontamination
R Frothingham et al. Biotechniques. 1992 Aug.
Abstract
UV irradiation is widely used to inactivate contaminating DNA in PCR. Highly UV-absorbent deoxyribonucleoside triphosphates in PCR mixtures reduce the efficiency of UV decontamination. Optimal decontamination may be achieved by irradiating the PCR mixture without the deoxyribonucleoside triphosphates.
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