MPI1, an essential gene encoding a mitochondrial membrane protein, is possibly involved in protein import into yeast mitochondria - PubMed (original) (raw)
MPI1, an essential gene encoding a mitochondrial membrane protein, is possibly involved in protein import into yeast mitochondria
A C Maarse et al. EMBO J. 1992 Oct.
Abstract
To identify components of the mitochondrial protein import pathway in yeast, we have adopted a positive selection procedure for isolating mutants disturbed in protein import. We have cloned and sequenced a gene, termed MPI1, that can rescue the genetic defect of one group of these mutants. MPI1 encodes a hydrophilic 48.8 kDa protein that is essential for cell viability. Mpi1p is a low abundance and constitutively expressed mitochondrial protein. Mpi1p is synthesized with a characteristic mitochondrial targeting sequence at its amino-terminus, which is most probably proteolytically removed during import. It is a membrane protein, oriented with its carboxy-terminus facing the intermembrane space. In cells depleted of Mpi1p activity, import of the precursor proteins that we tested thus far, is arrested. We speculate that the Mpi1 protein is a component of a proteinaceous import channel for translocation of precursor proteins across the mitochondrial inner membrane.
Similar articles
- Protein import into yeast mitochondria: the inner membrane import site protein ISP45 is the MPI1 gene product.
Horst M, Jenö P, Kronidou NG, Bolliger L, Oppliger W, Scherer P, Manning-Krieg U, Jascur T, Schatz G. Horst M, et al. EMBO J. 1993 Aug;12(8):3035-41. doi: 10.1002/j.1460-2075.1993.tb05972.x. EMBO J. 1993. PMID: 8344245 Free PMC article. - Identification of the essential yeast protein MIM17, an integral mitochondrial inner membrane protein involved in protein import.
Maarse AC, Blom J, Keil P, Pfanner N, Meijer M. Maarse AC, et al. FEBS Lett. 1994 Aug 1;349(2):215-21. doi: 10.1016/0014-5793(94)00669-5. FEBS Lett. 1994. PMID: 8050569 - SMS1, a high-copy suppressor of the yeast mas6 mutant, encodes an essential inner membrane protein required for mitochondrial protein import.
Ryan KR, Menold MM, Garrett S, Jensen RE. Ryan KR, et al. Mol Biol Cell. 1994 May;5(5):529-38. doi: 10.1091/mbc.5.5.529. Mol Biol Cell. 1994. PMID: 7919535 Free PMC article. - Mechanisms of protein translocation into mitochondria.
Voos W, Martin H, Krimmer T, Pfanner N. Voos W, et al. Biochim Biophys Acta. 1999 Nov 16;1422(3):235-54. doi: 10.1016/s0304-4157(99)00007-6. Biochim Biophys Acta. 1999. PMID: 10548718 Review. - Targeting of proteins to mitochondria.
Lithgow T. Lithgow T. FEBS Lett. 2000 Jun 30;476(1-2):22-6. doi: 10.1016/s0014-5793(00)01663-x. FEBS Lett. 2000. PMID: 10878243 Review.
Cited by
- TIMM44 is a potential therapeutic target of human glioma.
Guo YZ, Chen G, Huang M, Wang Y, Liu YY, Jiang Q, Cao C, Liu F. Guo YZ, et al. Theranostics. 2022 Oct 31;12(17):7586-7602. doi: 10.7150/thno.78616. eCollection 2022. Theranostics. 2022. PMID: 36438483 Free PMC article. - Towards a molecular mechanism underlying mitochondrial protein import through the TOM and TIM23 complexes.
Ford HC, Allen WJ, Pereira GC, Liu X, Dillingham MS, Collinson I. Ford HC, et al. Elife. 2022 Jun 8;11:e75426. doi: 10.7554/eLife.75426. Elife. 2022. PMID: 35674314 Free PMC article. - Interplay between Mitochondrial Protein Import and Respiratory Complexes Assembly in Neuronal Health and Degeneration.
Needs HI, Protasoni M, Henley JM, Prudent J, Collinson I, Pereira GC. Needs HI, et al. Life (Basel). 2021 May 11;11(5):432. doi: 10.3390/life11050432. Life (Basel). 2021. PMID: 34064758 Free PMC article. Review. - ALS/FTD mutations in UBQLN2 are linked to mitochondrial dysfunction through loss-of-function in mitochondrial protein import.
Lin BC, Phung TH, Higgins NR, Greenslade JE, Prado MA, Finley D, Karbowski M, Polster BM, Monteiro MJ. Lin BC, et al. Hum Mol Genet. 2021 Jun 17;30(13):1230-1246. doi: 10.1093/hmg/ddab116. Hum Mol Genet. 2021. PMID: 33891006 Free PMC article. - Adaptation of a Genetic Screen Reveals an Inhibitor for Mitochondrial Protein Import Component Tim44.
Miyata N, Tang Z, Conti MA, Johnson ME, Douglas CJ, Hasson SA, Damoiseaux R, Chang CA, Koehler CM. Miyata N, et al. J Biol Chem. 2017 Mar 31;292(13):5429-5442. doi: 10.1074/jbc.M116.770131. Epub 2017 Feb 6. J Biol Chem. 2017. PMID: 28167535 Free PMC article.
References
- Genetics. 1979 Sep;93(1):51-65 - PubMed
- Eur J Biochem. 1985 Feb 15;147(1):153-61 - PubMed
- Proc Natl Acad Sci U S A. 1979 Oct;76(10):5134-7 - PubMed
- Mol Cell Biol. 1991 Nov;11(11):5487-96 - PubMed
- EMBO J. 1991 Nov;10(11):3273-80 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases