The plant amino acid mimosine may inhibit initiation at origins of replication in Chinese hamster cells - PubMed (original) (raw)

The plant amino acid mimosine may inhibit initiation at origins of replication in Chinese hamster cells

P J Mosca et al. Mol Cell Biol. 1992 Oct.

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Abstract

An understanding of replication initiation in mammalian cells has been hampered by the lack of mutations and/or inhibitors that arrest cells just prior to entry into the S period. The plant amino acid mimosine has recently been suggested to inhibit cells at a regulatory step in late G1. We have examined the effects of mimosine on cell cycle traverse in the mimosine [corrected]-resistant CHO cell line CHOC 400. When administered to cultures for 14 h after reversal of a G0 block, the drug appears to arrest the population at the G1/S boundary, and upon its removal cells enter the S phase in a synchronous wave. However, when methotrexate is administered to an actively dividing asynchronous culture, cells are arrested not only at the G1/S interface but also in early and middle S phase. Most interestingly, two-dimensional gel analysis of replication intermediates in the initiation locus of the amplified dihydrofolate reductase domain suggests that mimosine may actually inhibit initiation. Thus, this drug represents a new class of inhibitors that may open a window on regulatory events occurring at individual origins of replication.

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References

    1. Annu Rev Biochem. 1991;60:513-52 - PubMed
    1. Proc Natl Acad Sci U S A. 1982 Jul;79(13):4083-7 - PubMed
    1. Cell. 1990 Sep 7;62(5):955-65 - PubMed
    1. Cell. 1989 Jun 16;57(6):909-20 - PubMed
    1. Mol Cell Biol. 1989 Feb;9(2):532-40 - PubMed

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