A mammalian factor that binds telomeric TTAGGG repeats in vitro - PubMed (original) (raw)

A mammalian factor that binds telomeric TTAGGG repeats in vitro

Z Zhong et al. Mol Cell Biol. 1992 Nov.

Abstract

We have identified a DNA-binding activity with specificity for the TTAGGG repeat arrays found at mammalian telomeres. This factor, called TTAGGG repeat factor (TRF), is present in nuclear extracts of human, mouse, and monkey cells. TRF from HeLa cells was characterized in detail by electrophoretic mobility shift assays. It binds double-stranded TTAGGG repeats in linear and circular DNAs. Single-stranded repeats are not recognized. The optimal site for TRF appears to contain more than six contiguous TTAGGG repeats. Tandem arrays of TAGGG, TTTAGGG, TTTTAGGG, TTGGGG, and TTAGGC repeats do not bind TRF well, indicating that TRF preferentially recognizes the telomeric repeat sequence present at mammalian chromosome ends. The apparent molecular mass of this factor, based on recovery of TRF from sodium dodecyl sulfate-polyacrylamide gels, is approximately 50 kDa. We suggest that TRF binds along the length of mammalian telomeres.

PubMed Disclaimer

Similar articles

• Structure, subnuclear distribution, and nuclear matrix association of the mammalian telomeric complex.
  Ludérus ME, van Steensel B, Chong L, Sibon OC, Cremers FF, de Lange T. Ludérus ME, et al. J Cell Biol. 1996 Nov;135(4):867-81. doi: 10.1083/jcb.135.4.867. J Cell Biol. 1996. PMID: 8922373 Free PMC article.
• A Xenopus egg factor with DNA-binding properties characteristic of terminus-specific telomeric proteins.
  Cardenas ME, Bianchi A, de Lange T. Cardenas ME, et al. Genes Dev. 1993 May;7(5):883-94. doi: 10.1101/gad.7.5.883. Genes Dev. 1993. PMID: 7684008
• The telobox, a Myb-related telomeric DNA binding motif found in proteins from yeast, plants and human.
  Bilaud T, Koering CE, Binet-Brasselet E, Ancelin K, Pollice A, Gasser SM, Gilson E. Bilaud T, et al. Nucleic Acids Res. 1996 Apr 1;24(7):1294-303. doi: 10.1093/nar/24.7.1294. Nucleic Acids Res. 1996. PMID: 8614633 Free PMC article.
• TRF1, a mammalian telomeric protein.
  Smith S, de Lange T. Smith S, et al. Trends Genet. 1997 Jan;13(1):21-6. doi: 10.1016/s0168-9525(96)10052-4. Trends Genet. 1997. PMID: 9009844 Review.
• At the Beginning of the End and in the Middle of the Beginning: Structure and Maintenance of Telomeric DNA Repeats and Interstitial Telomeric Sequences.
  Aksenova AY, Mirkin SM. Aksenova AY, et al. Genes (Basel). 2019 Feb 5;10(2):118. doi: 10.3390/genes10020118. Genes (Basel). 2019. PMID: 30764567 Free PMC article. Review.

Cited by

• Nuclear envelope budding inhibition slows down progerin-induced aging process.
  Wang X, Ma L, Lu D, Zhao G, Ren H, Lin Q, Jia M, Huang F, Wang S, Xu Z, Yang Z, Chu Y, Xu Z, Li W, Yu L, Jiang Q, Zhang C. Wang X, et al. Proc Natl Acad Sci U S A. 2024 Oct 8;121(41):e2321378121. doi: 10.1073/pnas.2321378121. Epub 2024 Oct 1. Proc Natl Acad Sci U S A. 2024. PMID: 39352925
• Structural basis of telomeric nucleosome recognition by shelterin factor TRF1.
  Hu H, van Roon AM, Ghanim GE, Ahsan B, Oluwole AO, Peak-Chew SY, Robinson CV, Nguyen THD. Hu H, et al. Sci Adv. 2023 Aug 25;9(34):eadi4148. doi: 10.1126/sciadv.adi4148. Epub 2023 Aug 25. Sci Adv. 2023. PMID: 37624885 Free PMC article.
• Single-molecule imaging of genome maintenance proteins encountering specific DNA sequences and structures.
  Irvin EM, Wang H. Irvin EM, et al. DNA Repair (Amst). 2023 Aug;128:103528. doi: 10.1016/j.dnarep.2023.103528. Epub 2023 Jun 24. DNA Repair (Amst). 2023. PMID: 37392578 Free PMC article. Review.
• Rap1 prevents fusions between long telomeres in fission yeast.
  Pan L, Tormey D, Bobon N, Baumann P. Pan L, et al. EMBO J. 2022 Oct 17;41(20):e110458. doi: 10.15252/embj.2021110458. Epub 2022 Sep 5. EMBO J. 2022. PMID: 36059259 Free PMC article.
• Regulation of Antigenic Variation by Trypanosoma brucei Telomere Proteins Depends on Their Unique DNA Binding Activities.
  Li B, Zhao Y. Li B, et al. Pathogens. 2021 Jul 30;10(8):967. doi: 10.3390/pathogens10080967. Pathogens. 2021. PMID: 34451431 Free PMC article. Review.

References

1. 1. Nucleic Acids Res. 1989 Apr 25;17(8):3247-60 - PubMed
2. 1. Annu Rev Genet. 1989;23:579-604 - PubMed
3. 1. Proc Natl Acad Sci U S A. 1989 Sep;86(18):7049-53 - PubMed
4. 1. Mol Cell Biol. 1989 Jan;9(1):345-8 - PubMed
5. 1. Cell. 1988 Apr 22;53(2):211-7 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central
  • Taylor & Francis

• Other Literature Sources

  • The Lens - Patent Citations Database

• Molecular Biology Databases

  • BioCyc
  • Gene Ontology