Cloning of mycobacterial histidine synthesis genes by complementation of a Mycobacterium smegmatis auxotroph - PubMed (original) (raw)

Cloning of mycobacterial histidine synthesis genes by complementation of a Mycobacterium smegmatis auxotroph

S Hinshelwood et al. Mol Microbiol. 1992 Oct.

Abstract

Histidine-requiring auxotrophs of Mycobacterium smegmatis were isolated following N-methyl-N'-nitro-N-nitrosoguanidine treatment. One of these mutants, his5, was transformed with an M. smegmatis shuttle cosmid library, and complementing clones were isolated at a frequency of approximately 1%. A 2.3 kb fragment was subcloned and sequenced, and found to contain the start of an operon including the hisD gene and part of the hisC gene. No hisG gene was detected upstream of hisD, suggesting that the regulation of histidine biosynthesis in mycobacteria may differ from that of Escherichia coli. The strategy used here will allow the molecular genetics of complex mycobacterial-specific biosynthetic pathways involved in the virulence of pathogenic species to be studied.

PubMed Disclaimer

Similar articles

• Cloning of the histidine biosynthetic genes from Corynebacterium glutamicum: organization and analysis of the hisG and hisE genes.
  Kwon JH, Chun JY, Lee HS, Cheon CI, Song ES, Min KH, Lee MS. Kwon JH, et al. Can J Microbiol. 2000 Sep;46(9):848-55. Can J Microbiol. 2000. PMID: 11006846
• Identification of genes involved in the sequestration of iron in mycobacteria: the ferric exochelin biosynthetic and uptake pathways.
  Fiss EH, Yu S, Jacobs WR Jr. Fiss EH, et al. Mol Microbiol. 1994 Nov;14(3):557-69. doi: 10.1111/j.1365-2958.1994.tb02189.x. Mol Microbiol. 1994. PMID: 7885234
• Isolation and characterization of the aspartokinase and aspartate semialdehyde dehydrogenase operon from mycobacteria.
  Cirillo JD, Weisbrod TR, Pascopella L, Bloom BR, Jacobs WR Jr. Cirillo JD, et al. Mol Microbiol. 1994 Feb;11(4):629-39. doi: 10.1111/j.1365-2958.1994.tb00342.x. Mol Microbiol. 1994. PMID: 7910936
• Regulation of the histidine operon: translation-controlled transcription termination (a mechanism common to several biosynthetic operons).
  Blasi F, Bruni CB. Blasi F, et al. Curr Top Cell Regul. 1981;19:1-45. doi: 10.1016/b978-0-12-152819-5.50018-x. Curr Top Cell Regul. 1981. PMID: 6277571 Review. No abstract available.
• [Systems of genes and proteins affecting mycobacteria virulence and their homologs participation in conjugation of mycobacterium smegmatis].
  Prozorov AA, Zaichikova MV, Danilenko VN. Prozorov AA, et al. Genetika. 2013 Jan;49(1):125-41. doi: 10.7868/s0016675813010098. Genetika. 2013. PMID: 23662430 Review. Russian.

Cited by

• Mycobacterium tuberculosis metabolism.
  Warner DF. Warner DF. Cold Spring Harb Perspect Med. 2014 Dec 11;5(4):a021121. doi: 10.1101/cshperspect.a021121. Cold Spring Harb Perspect Med. 2014. PMID: 25502746 Free PMC article. Review.
• Mycobacterium tuberculosis and Clostridium difficille interactomes: demonstration of rapid development of computational system for bacterial interactome prediction.
  Ananthasubramanian S, Metri R, Khetan A, Gupta A, Handen A, Chandra N, Ganapathiraju M. Ananthasubramanian S, et al. Microb Inform Exp. 2012 Mar 21;2:4. doi: 10.1186/2042-5783-2-4. Microb Inform Exp. 2012. PMID: 22587966 Free PMC article.
• Role of the DinB homologs Rv1537 and Rv3056 in Mycobacterium tuberculosis.
  Kana BD, Abrahams GL, Sung N, Warner DF, Gordhan BG, Machowski EE, Tsenova L, Sacchettini JC, Stoker NG, Kaplan G, Mizrahi V. Kana BD, et al. J Bacteriol. 2010 Apr;192(8):2220-7. doi: 10.1128/JB.01135-09. Epub 2010 Feb 5. J Bacteriol. 2010. PMID: 20139184 Free PMC article.
• Mycobacteria: bugs and bugbears (two steps forward and one step back).
  Parish T, Stoker NG. Parish T, et al. Mol Biotechnol. 1999 Dec 15;13(3):191-200. doi: 10.1385/MB:13:3:191. Mol Biotechnol. 1999. PMID: 10934532 Review.
• Genetic antagonism and hypermutability in Mycobacterium smegmatis.
  Karunakaran P, Davies J. Karunakaran P, et al. J Bacteriol. 2000 Jun;182(12):3331-5. doi: 10.1128/JB.182.12.3331-3335.2000. J Bacteriol. 2000. PMID: 10852861 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley