Airway eosinophils accumulate in the mediastinal lymph nodes but lack antigen-presenting potential for naive T cells - PubMed (original) (raw)
Comparative Study
. 2003 Oct 1;171(7):3372-8.
doi: 10.4049/jimmunol.171.7.3372.
Affiliations
- PMID: 14500630
- DOI: 10.4049/jimmunol.171.7.3372
Comparative Study
Airway eosinophils accumulate in the mediastinal lymph nodes but lack antigen-presenting potential for naive T cells
Leonie S van Rijt et al. J Immunol. 2003.
Abstract
Asthma is characterized by infiltration of the airway wall with eosinophils. Although eosinophils are considered to be effector cells, recent studies have reported their ability to activate primed Th2 cells. In this study, we investigated whether eosinophils are capable of presenting Ag to unprimed T cells in draining lymph nodes (DLN) of the lung and compared this capacity with professional dendritic cells (DC). During development of eosinophilic airway inflammation in OVA-sensitized and challenged mice, CCR3(+) eosinophils accumulated in the DLN. To study their function, eosinophils were isolated from the bronchoalveolar lavage fluid of mice by sorting on CCR3(+)B220(-)CD3(-)CD11c(dim) low autofluorescent cells, avoiding contamination with other APCs, and were intratracheally injected into mice that previously received CFSE-labeled OVA TCR-transgenic T cells. Eosinophils did not induce divisions of T cells in the DLN, whereas DC induced on average 3.7 divisions in 45.7% of T cells. To circumvent the need for Ag processing or migration in vivo, eosinophils were pulsed with OVA peptide and were still not able to induce T cell priming in vitro, whereas DC induced vigorous proliferation. This lack of Ag-presenting ability was explained by the very weak expression of MHC class II on fresh eosinophils, despite expression of the costimulatory molecules CD80 and ICAM-1. This investigation does not support any role for airway eosinophils as APCs to naive T cells, despite their migration to the DLN at times of allergen exposure. DC are clearly superior in activating T cells in the DLN of the lung.
Similar articles
- Airway eosinophils: allergic inflammation recruited professional antigen-presenting cells.
Wang HB, Ghiran I, Matthaei K, Weller PF. Wang HB, et al. J Immunol. 2007 Dec 1;179(11):7585-92. doi: 10.4049/jimmunol.179.11.7585. J Immunol. 2007. PMID: 18025204 Free PMC article. - Differential capacity of CD8+ alpha or CD8- alpha dendritic cell subsets to prime for eosinophilic airway inflammation in the T-helper type 2-prone milieu of the lung.
Hammad H, de Vries VC, Maldonado-Lopez R, Moser M, Maliszewski C, Hoogsteden HC, Lambrecht BN. Hammad H, et al. Clin Exp Allergy. 2004 Dec;34(12):1834-40. doi: 10.1111/j.1365-2222.2004.02133.x. Clin Exp Allergy. 2004. PMID: 15663556 - Pulmonary eosinophils express HLA-DR in chronic eosinophilic pneumonia.
Beninati W, Derdak S, Dixon PF, Grider DJ, Strollo DC, Hensley RE, Lucey DR. Beninati W, et al. J Allergy Clin Immunol. 1993 Sep;92(3):442-9. doi: 10.1016/0091-6749(93)90123-w. J Allergy Clin Immunol. 1993. PMID: 8360395 Review. - Eosinophils as antigen-presenting cells in allergic upper airway disease.
Akuthota P, Wang H, Weller PF. Akuthota P, et al. Curr Opin Allergy Clin Immunol. 2010 Feb;10(1):14-9. doi: 10.1097/ACI.0b013e328334f693. Curr Opin Allergy Clin Immunol. 2010. PMID: 19949323 Free PMC article. Review.
Cited by
- Eosinophils: Nemeses of Pulmonary Pathogens?
LeMessurier KS, Samarasinghe AE. LeMessurier KS, et al. Curr Allergy Asthma Rep. 2019 Jun 19;19(8):36. doi: 10.1007/s11882-019-0867-1. Curr Allergy Asthma Rep. 2019. PMID: 31218528 Free PMC article. Review. - Dendritic Cell Activating Receptor 1 (DCAR1) Associates With FcεRIγ and Is Expressed by Myeloid Cell Subsets in the Rat.
Daws MR, Nakken B, Lobato-Pascual A, Josien R, Dissen E, Fossum S. Daws MR, et al. Front Immunol. 2019 May 10;10:1060. doi: 10.3389/fimmu.2019.01060. eCollection 2019. Front Immunol. 2019. PMID: 31134097 Free PMC article. - Professional and 'Amateur' Antigen-Presenting Cells In Type 2 Immunity.
Schuijs MJ, Hammad H, Lambrecht BN. Schuijs MJ, et al. Trends Immunol. 2019 Jan;40(1):22-34. doi: 10.1016/j.it.2018.11.001. Epub 2018 Nov 27. Trends Immunol. 2019. PMID: 30502024 Free PMC article. Review. - A pathophysiological role of PDE3 in allergic airway inflammation.
Beute J, Lukkes M, Koekoek EP, Nastiti H, Ganesh K, de Bruijn MJ, Hockman S, van Nimwegen M, Braunstahl GJ, Boon L, Lambrecht BN, Manganiello VC, Hendriks RW, KleinJan A. Beute J, et al. JCI Insight. 2018 Jan 25;3(2):e94888. doi: 10.1172/jci.insight.94888. eCollection 2018 Jan 25. JCI Insight. 2018. PMID: 29367458 Free PMC article. - Granulocytes: New Members of the Antigen-Presenting Cell Family.
Lin A, Loré K. Lin A, et al. Front Immunol. 2017 Dec 11;8:1781. doi: 10.3389/fimmu.2017.01781. eCollection 2017. Front Immunol. 2017. PMID: 29321780 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous