Bone marrow monocyte lineage cells adhere on injured endothelium in a monocyte chemoattractant protein-1-dependent manner and accelerate reendothelialization as endothelial progenitor cells - PubMed (original) (raw)
. 2003 Nov 14;93(10):980-9.
doi: 10.1161/01.RES.0000099245.08637.CE. Epub 2003 Oct 2.
Katsuya Amano, Kazutaka Uehira, Masayuki Yoshida, Yasunobu Nishiwaki, Yoshihisa Nozawa, Denan Jin, Shinji Takai, Mizuo Miyazaki, Kensuke Egashira, Takayuki Imada, Toshiji Iwasaka, Hiroaki Matsubara
Affiliations
- PMID: 14525810
- DOI: 10.1161/01.RES.0000099245.08637.CE
Bone marrow monocyte lineage cells adhere on injured endothelium in a monocyte chemoattractant protein-1-dependent manner and accelerate reendothelialization as endothelial progenitor cells
Soichiro Fujiyama et al. Circ Res. 2003.
Abstract
Peripheral blood (PB)-derived CD14+ monocytes were shown to transdifferentiate into endothelial cell (EC) lineage cells and contribute to neovascularization. We investigated whether bone marrow (BM)- or PB-derived CD34-/CD14+ cells are involved in reendothelialization after carotid balloon injury. Although neither hematopoietic nor mesenchymal stem cells were included in human BM-derived CD34-/CD14+ monocyte lineage cells (BM-MLCs), they expressed EC-specific markers (Tie2, CD31, VE-cadherin, and endoglin) to an extent identical to mature ECs. When BM-MLCs were cultured with vascular endothelial growth factors, hematopoietic markers were drastically decreased and new EC-specific markers (Flk and CD34) were induced. BM-MLCs were intra-arterially transplanted into balloon-injured arteries of athymic nude rats. When BM-MLCs were activated by monocyte chemoattractant protein-1 (MCP-1) in vivo or in vitro, they adhered onto injured endothelium, differentiated into EC-like cells by losing hematopoietic markers, and inhibited neointimal hyperplasia. Ability to prevent neointimal hyperplasia was more efficient than that of BM-derived CD34+ cells. MCP-dependent adhesion was not observed in PB-derived CD34-/CD14+ monocytes. Regenerated endothelium exhibited a cobblestone appearance, blocked extravasation of dye, and induced NO-dependent vasorelaxation. Basal adhesive activities on HUVECs under laminar flow and beta1-integrin expression (basal and active forms) were significantly increased in BM-MLCs compared with PB-derived monocytes. MCP-1 markedly enhanced adhesive activity of BM-MLCs (2.8-fold) on HUVECs by activating beta1-integrin conformation. Thus, BM-MLCs can function as EC progenitors that are more potent than CD34+ cells and acquire the ability to adhere on injured endothelium in a MCP-1-dependent manner, leading to reendothelialization associated with inhibition of intimal hyperplasia. This will open a novel window to MCP-1-mediated biological actions and vascular regeneration strategies by cell therapy.
Similar articles
- Rosiglitazone facilitates angiogenic progenitor cell differentiation toward endothelial lineage: a new paradigm in glitazone pleiotropy.
Wang CH, Ciliberti N, Li SH, Szmitko PE, Weisel RD, Fedak PW, Al-Omran M, Cherng WJ, Li RK, Stanford WL, Verma S. Wang CH, et al. Circulation. 2004 Mar 23;109(11):1392-400. doi: 10.1161/01.CIR.0000123231.49594.21. Epub 2004 Mar 1. Circulation. 2004. PMID: 14993120 - Endogenous NO blockade enhances tissue factor expression via increased Ca2+ influx through MCP-1 in endothelial cells by monocyte adhesion.
Sakamoto T, Ishibashi T, Sakamoto N, Sugimoto K, Egashira K, Ohkawara H, Nagata K, Yokoyama K, Kamioka M, Ichiki T, Sugimoto N, Kurabayashi M, Suzuki K, Takuwa Y, Maruyama Y. Sakamoto T, et al. Arterioscler Thromb Vasc Biol. 2005 Sep;25(9):2005-11. doi: 10.1161/01.ATV.0000178171.61754.cd. Epub 2005 Jul 14. Arterioscler Thromb Vasc Biol. 2005. PMID: 16020745 - Granulocyte colony-stimulating factor-mobilized circulating c-Kit+/Flk-1+ progenitor cells regenerate endothelium and inhibit neointimal hyperplasia after vascular injury.
Takamiya M, Okigaki M, Jin D, Takai S, Nozawa Y, Adachi Y, Urao N, Tateishi K, Nomura T, Zen K, Ashihara E, Miyazaki M, Tatsumi T, Takahashi T, Matsubara H. Takamiya M, et al. Arterioscler Thromb Vasc Biol. 2006 Apr;26(4):751-7. doi: 10.1161/01.ATV.0000205607.98538.9a. Epub 2006 Jan 26. Arterioscler Thromb Vasc Biol. 2006. PMID: 16439710 - Endothelial progenitor cells: characterization and role in vascular biology.
Urbich C, Dimmeler S. Urbich C, et al. Circ Res. 2004 Aug 20;95(4):343-53. doi: 10.1161/01.RES.0000137877.89448.78. Circ Res. 2004. PMID: 15321944 Review. - [Cell cycle-dependent change of the adhesive character of CD34+ progenitor cells and their VLA-4 expression].
Yamaguchi M, Ikebuchi K, Hirayama F, Sato N, Sekiguchi S. Yamaguchi M, et al. Rinsho Byori. 1999 May;47(5):439-46. Rinsho Byori. 1999. PMID: 10375965 Review. Japanese.
Cited by
- Innovative Treatments to Counteract Endothelial Dysfunction in Chronic Kidney Disease Patients.
Marrone G, Cornali K, Di Lauro M, Ceravolo MJ, Di Marco L, Manca di Villahermosa S, Mitterhofer AP, Noce A. Marrone G, et al. Biomedicines. 2024 May 14;12(5):1085. doi: 10.3390/biomedicines12051085. Biomedicines. 2024. PMID: 38791047 Free PMC article. Review. - Interrogations of single-cell RNA splicing landscapes with SCASL define new cell identities with physiological relevance.
Xiang X, He Y, Zhang Z, Yang X. Xiang X, et al. Nat Commun. 2024 Mar 9;15(1):2164. doi: 10.1038/s41467-024-46480-9. Nat Commun. 2024. PMID: 38461306 Free PMC article. - Vascular Aging: Assessment and Intervention.
Li A, Yan J, Zhao Y, Yu Z, Tian S, Khan AH, Zhu Y, Wu A, Zhang C, Tian XL. Li A, et al. Clin Interv Aging. 2023 Aug 17;18:1373-1395. doi: 10.2147/CIA.S423373. eCollection 2023. Clin Interv Aging. 2023. PMID: 37609042 Free PMC article. Review. - Role of Angiopoietic Coronary Endothelial Dysfunction in the Pathogenesis of Ischemic Cardiomyopathy.
Chumakova SP, Urazova OI, Shipulin VM, Andreev SL, Denisenko OA, Gladkovskaya MV, Litvinova LS, Bubenchikov MA. Chumakova SP, et al. Biomedicines. 2023 Jul 10;11(7):1950. doi: 10.3390/biomedicines11071950. Biomedicines. 2023. PMID: 37509589 Free PMC article. - Promoting Angiogenesis Using Immune Cells for Tissue-Engineered Vascular Grafts.
Wang L, Wei X, Wang Y. Wang L, et al. Ann Biomed Eng. 2023 Apr;51(4):660-678. doi: 10.1007/s10439-023-03158-5. Epub 2023 Feb 11. Ann Biomed Eng. 2023. PMID: 36774426 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials
Miscellaneous