Coupling gene chip analyses and rat genetic variances in identifying potential target genes that may contribute to neuropathic allodynia development - PubMed (original) (raw)
Coupling gene chip analyses and rat genetic variances in identifying potential target genes that may contribute to neuropathic allodynia development
Carolina R Valder et al. J Neurochem. 2003 Nov.
Free article
Abstract
Genetic factors and nerve injury-induced changes of gene expression in sensory neurons are potential contributors to tactile allodynia, a neuropathic pain state manifested as hypersensitivity to innocuous mechanical stimulation. To uncover genes relevant to neuropathic allodynia, we analyzed gene expression profiles in dorsal root ganglia (DRG) of spinal nerve-ligated Harlan and Holtzman Sprague Dawley rats, strains with different susceptibilities to neuropathic allodynia. Using Affymetrix gene chips, we identified genes showing differential basal-level expression in these strains without injury-induced regulation. Of more than 8000 genes analyzed, less than 180 genes in each strain were regulated after injury, and 19-22% of that was regulated in a strain-specific manner. Importantly, we identified functionally related genes that were co-regulated post injury in one or both strains. In situ hybridization and real-time PCR analyses of a subset of identified genes confirmed the patterns of the microarray data, and the former also demonstrated that injury-induced changes occurred, not only in neurons, but also in non-neuronal cells. Together, our studies provide a global view of injury plasticity in DRG of these rat stains and support a plasticity-based mechanism mediating variations in allodynia susceptibility, thus providing a source for further characterization of neuropathic pain-relevant genes and potential pathways.
Similar articles
- Identification of lncRNA expression profile in the spinal cord of mice following spinal nerve ligation-induced neuropathic pain.
Jiang BC, Sun WX, He LN, Cao DL, Zhang ZJ, Gao YJ. Jiang BC, et al. Mol Pain. 2015 Jul 17;11:43. doi: 10.1186/s12990-015-0047-9. Mol Pain. 2015. PMID: 26184882 Free PMC article. - Dorsal horn-enriched genes identified by DNA microarray, in situ hybridization and immunohistochemistry.
Sun H, Xu J, Della Penna KB, Benz RJ, Kinose F, Holder DJ, Koblan KS, Gerhold DL, Wang H. Sun H, et al. BMC Neurosci. 2002 Aug 20;3:11. doi: 10.1186/1471-2202-3-11. BMC Neurosci. 2002. PMID: 12188929 Free PMC article. - Regulation of two rat mas-related genes in a model of neuropathic pain.
Gustafson EL, Maguire M, Campanella M, Tarozzo G, Jia Y, Dong XW, Laverty M, Murgolo N, Priestley T, Reggiani A, Monsma F, Beltramo M. Gustafson EL, et al. Brain Res Mol Brain Res. 2005 Dec 7;142(1):58-64. doi: 10.1016/j.molbrainres.2005.09.014. Epub 2005 Oct 24. Brain Res Mol Brain Res. 2005. PMID: 16246453 - Genetic studies of human neuropathic pain conditions: a review.
Zorina-Lichtenwalter K, Parisien M, Diatchenko L. Zorina-Lichtenwalter K, et al. Pain. 2018 Mar;159(3):583-594. doi: 10.1097/j.pain.0000000000001099. Pain. 2018. PMID: 29240606 Free PMC article. Review.
Cited by
- Cooperativity between remote sites of ectopic spiking allows afterdischarge to be initiated and maintained at different locations.
Coggan JS, Sejnowski TJ, Prescott SA. Coggan JS, et al. J Comput Neurosci. 2015 Aug;39(1):17-28. doi: 10.1007/s10827-015-0562-8. Epub 2015 May 1. J Comput Neurosci. 2015. PMID: 25929191 Free PMC article. - Stem cell therapy for neuropathic pain treatment.
Siniscalco D, Rossi F, Maione S. Siniscalco D, et al. J Stem Cells Regen Med. 2007 Nov 14;3(1):2-11. doi: 10.46582/jsrm.0301002. eCollection 2007. J Stem Cells Regen Med. 2007. PMID: 24693013 Free PMC article. - Cyclic nucleotide signaling in sensory neuron hyperexcitability and chronic pain after nerve injury.
Li ZH, Cui D, Qiu CJ, Song XJ. Li ZH, et al. Neurobiol Pain. 2019 Mar 8;6:100028. doi: 10.1016/j.ynpai.2019.100028. eCollection 2019 Aug-Dec. Neurobiol Pain. 2019. PMID: 31223142 Free PMC article. Review. - Application of pulsed radiofrequency currents to rat dorsal root ganglia modulates nerve injury-induced tactile allodynia.
Perret DM, Kim DS, Li KW, Sinavsky K, Newcomb RL, Miller JM, Luo ZD. Perret DM, et al. Anesth Analg. 2011 Sep;113(3):610-6. doi: 10.1213/ANE.0b013e31821e974f. Epub 2011 May 19. Anesth Analg. 2011. PMID: 21596869 Free PMC article. - Painful nerve injury upregulates thrombospondin-4 expression in dorsal root ganglia.
Pan B, Yu H, Park J, Yu YP, Luo ZD, Hogan QH. Pan B, et al. J Neurosci Res. 2015 Mar;93(3):443-53. doi: 10.1002/jnr.23498. Epub 2014 Oct 18. J Neurosci Res. 2015. PMID: 25327416 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical