Activin inhibits binding of transcription factor Pit-1 to the growth hormone promoter - PubMed (original) (raw)
Activin inhibits binding of transcription factor Pit-1 to the growth hormone promoter
R S Struthers et al. Proc Natl Acad Sci U S A. 1992.
Abstract
Activin A is a potent growth and differentiation factor related to transforming growth factor beta. In somatotrophs, activin suppresses the biosynthesis and secretion of growth hormone (GH) and cellular proliferation. We report here that, in MtTW15 somatotrophic tumor cells, activin decreased GH mRNA levels and inhibited expression of transfected GH promoter--chloramphenicol acetyltransferase fusion genes. Deletion mapping of nucleotide sequences mediating this inhibition led to the identification of a region that has previously been characterized as binding the pituitary-specific transcription factor Pit-1/GHF-1. Characterization of nuclear factor binding to this region demonstrated that binding of Pit-1 to the GH promoter is lost on activin treatment. These results indicate that activin-induced repression of GH biosynthesis is mediated by the loss of tissue-specific transcription factor binding to the GH promoter and suggest a possible general mechanism for other activin responses, whereby activin regulates the function of other POU- or homeodomain-containing transcription factors.
Similar articles
- Activin increases phosphorylation and decreases stability of the transcription factor Pit-1 in MtTW15 somatotrope cells.
Gaddy-Kurten D, Vale WW. Gaddy-Kurten D, et al. J Biol Chem. 1995 Dec 1;270(48):28733-9. doi: 10.1074/jbc.270.48.28733. J Biol Chem. 1995. PMID: 7499395 - Synergistic activation of the rat growth hormone promoter by Pit-1 and the thyroid hormone receptor.
Schaufele F, West BL, Baxter JD. Schaufele F, et al. Mol Endocrinol. 1992 Apr;6(4):656-65. doi: 10.1210/mend.6.4.1584227. Mol Endocrinol. 1992. PMID: 1584227 - Control of growth hormone synthesis.
Tuggle CK, Trenkle A. Tuggle CK, et al. Domest Anim Endocrinol. 1996 Jan;13(1):1-33. doi: 10.1016/0739-7240(95)00059-3. Domest Anim Endocrinol. 1996. PMID: 8625613 Review. - Pit-1 and Pit-2 role in growth hormone gene regulation.
Vila V, Jiménez O, Güell A, Castrillo JL. Vila V, et al. J Pediatr Endocrinol. 1993 Jul-Dec;6(3-4):225-8. doi: 10.1515/jpem.1993.6.3-4.225. J Pediatr Endocrinol. 1993. PMID: 7920986 Review.
Cited by
- Expression and localization of inhibin/activin and activin receptors in GH3 cells, a rat pituitary adenocarcinoma cell line.
Ying C, Zhang Z, Huang G, Li SQ, Ying SY. Ying C, et al. J Endocrinol Invest. 1996 Jan;19(1):6-11. doi: 10.1007/BF03347851. J Endocrinol Invest. 1996. PMID: 8851685 - A Comparative Update on the Neuroendocrine Regulation of Growth Hormone in Vertebrates.
Vélez EJ, Unniappan S. Vélez EJ, et al. Front Endocrinol (Lausanne). 2021 Feb 23;11:614981. doi: 10.3389/fendo.2020.614981. eCollection 2020. Front Endocrinol (Lausanne). 2021. PMID: 33708174 Free PMC article. Review. - Setting limits on homeotic gene function: restraint of Sex combs reduced activity by teashirt and other homeotic genes.
Andrew DJ, Horner MA, Petitt MG, Smolik SM, Scott MP. Andrew DJ, et al. EMBO J. 1994 Mar 1;13(5):1132-44. doi: 10.1002/j.1460-2075.1994.tb06362.x. EMBO J. 1994. PMID: 7907545 Free PMC article.
References
- Science. 1991 Aug 16;253(5021):786-9 - PubMed
- Methods Enzymol. 1983;103:565-77 - PubMed
- Science. 1991 Jul 12;253(5016):197-9 - PubMed
- Nature. 1991 Apr 18;350(6319):622-4 - PubMed
- Proc Soc Exp Biol Med. 1991 Oct;198(1):500-12 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases