Conserved cis-regulatory modules mediate complex neural expression patterns of the eyeless gene in the Drosophila brain - PubMed (original) (raw)
. 2003 Oct;120(10):1113-26.
doi: 10.1016/j.mod.2003.08.007.
Affiliations
- PMID: 14568101
- DOI: 10.1016/j.mod.2003.08.007
Free article
Conserved cis-regulatory modules mediate complex neural expression patterns of the eyeless gene in the Drosophila brain
Yoshitsugu Adachi et al. Mech Dev. 2003 Oct.
Free article
Abstract
The Drosophila Pax-6 homologs eyeless (ey) and twin of eyeless (toy) are expressed in the eyes and in the central nervous system (CNS). In addition to the pivotal functions in eye development, previous studies revealed that ey also plays important roles in axonal development of the mushroom bodies, centers for associative learning and memory. It has been reported that a second intron enhancer that contains several Pax-6 binding sites mainly controls the eye-specific expression, but the DNA sequences that control CNS expression are unknown. In this work, we have dissected transcriptional enhancer elements of the ey gene that are required for the CNS expression in various developmental stages. We first show that CNS expression is independent of the eye-specific enhancer of the second intron. By systematic reporter studies, we have identified several discrete DNA elements in the 5' upstream region and in the second intron that cooperatively interact to generate most of the ey expression pattern in the CNS. DNA sequence comparison between the ey genes of distant Drosophila species has identified conserved modules that might be bound by the upstream regulatory factors of the ey gene in CNS development. Furthermore, by RNA interference and mutant studies, we show that ey expression in the brain is independent of the activity of toy and ey itself whereas in the eye primordia it requires both, supporting the notion that ey and toy are regulated by parallel and independent regulatory cascades in brain development.
Similar articles
- twin of eyeless, a second Pax-6 gene of Drosophila, acts upstream of eyeless in the control of eye development.
Czerny T, Halder G, Kloter U, Souabni A, Gehring WJ, Busslinger M. Czerny T, et al. Mol Cell. 1999 Mar;3(3):297-307. doi: 10.1016/s1097-2765(00)80457-8. Mol Cell. 1999. PMID: 10198632 - Functional analysis of an eye specific enhancer of the eyeless gene in Drosophila.
Hauck B, Gehring WJ, Walldorf U. Hauck B, et al. Proc Natl Acad Sci U S A. 1999 Jan 19;96(2):564-9. doi: 10.1073/pnas.96.2.564. Proc Natl Acad Sci U S A. 1999. PMID: 9892673 Free PMC article. - Probing the Drosophila retinal determination gene network in Tribolium (II): The Pax6 genes eyeless and twin of eyeless.
Yang X, Weber M, Zarinkamar N, Posnien N, Friedrich F, Wigand B, Beutel R, Damen WG, Bucher G, Klingler M, Friedrich M. Yang X, et al. Dev Biol. 2009 Sep 1;333(1):215-27. doi: 10.1016/j.ydbio.2009.06.013. Epub 2009 Jun 13. Dev Biol. 2009. PMID: 19527703 - Vertebrate eye development as modeled in Drosophila.
Wawersik S, Maas RL. Wawersik S, et al. Hum Mol Genet. 2000 Apr 12;9(6):917-25. doi: 10.1093/hmg/9.6.917. Hum Mol Genet. 2000. PMID: 10767315 Review. - Interplay of Pax6 and SOX2 in lens development as a paradigm of genetic switch mechanisms for cell differentiation.
Kondoh H, Uchikawa M, Kamachi Y. Kondoh H, et al. Int J Dev Biol. 2004;48(8-9):819-27. doi: 10.1387/ijdb.041868hk. Int J Dev Biol. 2004. PMID: 15558474 Review.
Cited by
- Knockout of a single Pax6 gene (toy but not ey) leads to compound eye deficiency and small head in honeybees.
Hu X, Cheng F, Gong Z, Qin K, Shan T, Li W, Zhang L, Yan W, Zeng Z, Wang Z. Hu X, et al. Commun Biol. 2024 Oct 14;7(1):1319. doi: 10.1038/s42003-024-07016-5. Commun Biol. 2024. PMID: 39402171 Free PMC article. - Mutations in abnormal spindle disrupt temporal transcription factor expression and trigger immune responses in the Drosophila brain.
Mannino MC, Cassidy MB, Florez S, Rusan Z, Chakraborty S, Schoborg T. Mannino MC, et al. Genetics. 2023 Dec 6;225(4):iyad188. doi: 10.1093/genetics/iyad188. Genetics. 2023. PMID: 37831641 Free PMC article. - A circular RNA Edis-Relish-castor axis regulates neuronal development in Drosophila.
Liu W, Liang W, Xiong XP, Li JL, Zhou R. Liu W, et al. PLoS Genet. 2022 Oct 27;18(10):e1010433. doi: 10.1371/journal.pgen.1010433. eCollection 2022 Oct. PLoS Genet. 2022. PMID: 36301831 Free PMC article. - Genome-wide analysis identifies Homothorax and Extradenticle as regulators of insulin in Drosophila Insulin-Producing cells.
Winant M, Buhler K, Clements J, De Groef S, Hens K, Vulsteke V, Callaerts P. Winant M, et al. PLoS Genet. 2022 Sep 12;18(9):e1010380. doi: 10.1371/journal.pgen.1010380. eCollection 2022 Sep. PLoS Genet. 2022. PMID: 36095003 Free PMC article. - Genomic organization of the autonomous regulatory domain of eyeless locus in Drosophila melanogaster.
Verma S, Pathak RU, Mishra RK. Verma S, et al. G3 (Bethesda). 2021 Dec 8;11(12):jkab338. doi: 10.1093/g3journal/jkab338. G3 (Bethesda). 2021. PMID: 34570231 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases