Roles of catalase and hydrogen peroxide in green tea polyphenol-induced chemopreventive effects - PubMed (original) (raw)
. 2004 Jan;308(1):317-23.
doi: 10.1124/jpet.103.058891. Epub 2003 Oct 20.
Affiliations
- PMID: 14569057
- DOI: 10.1124/jpet.103.058891
Roles of catalase and hydrogen peroxide in green tea polyphenol-induced chemopreventive effects
Tetsuya Yamamoto et al. J Pharmacol Exp Ther. 2004 Jan.
Abstract
The green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG) possesses promising anticancer potential. Although in vivo studies unveiled the metabolic routes and pharmacokinetics of EGCG and showed no adverse effects, in vitro studies at high concentrations demonstrated oxidative stress. EGCG causes differential oxidative environments in tumor versus normal epithelial cells, but the roles that EGCG, hydrogen peroxide (H2O2), and intracellular catalase play in the epithelial system are largely unknown. The current study employed enzyme activity assays, reactive oxygen species quantification, and immunoblotting to investigate whether EGCG-induced differential effects correlate with levels of key antioxidant enzymes and H2O2. It was found that normal human keratinocytes with high catalase activity are least susceptible to H2O2, whereas H2O2 caused significant cytotoxicity in oral carcinoma cell lines. However, the EGCG-induced differential effects could not be duplicated by H2O2 alone. The addition of exogenous catalase failed to completely prevent the EGCG-induced cytotoxicity and rescue the EGCG-induced growth arrest in the tumor cells. The antioxidant N-acetyl-L-cysteine rescued the tumor cells from H2O2-induced damage only, but not from EGCG-induced mitochondrial damage. Finally, alterations in catalase or superoxide dismutase activities were not observed upon EGCG exposure. In conclusion, although endogenous catalase may play a role in response to H2O2-induced cytotoxicity, the EGCG-induced cytotoxic effects on tumor cells mainly result from sources other than H2O2.
Similar articles
- Prooxidative effects of green tea polyphenol (-)-epigallocatechin-3-gallate on the HIT-T15 pancreatic beta cell line.
Suh KS, Chon S, Oh S, Kim SW, Kim JW, Kim YS, Woo JT. Suh KS, et al. Cell Biol Toxicol. 2010 Jun;26(3):189-99. doi: 10.1007/s10565-009-9137-7. Epub 2009 Sep 12. Cell Biol Toxicol. 2010. PMID: 19757103 - Epigallocatechin-3-gallate-induced stress signals in HT-29 human colon adenocarcinoma cells.
Chen C, Shen G, Hebbar V, Hu R, Owuor ED, Kong AN. Chen C, et al. Carcinogenesis. 2003 Aug;24(8):1369-78. doi: 10.1093/carcin/bgg091. Epub 2003 Jun 19. Carcinogenesis. 2003. PMID: 12819184 - Green tea component, catechin, induces apoptosis of human malignant B cells via production of reactive oxygen species.
Nakazato T, Ito K, Ikeda Y, Kizaki M. Nakazato T, et al. Clin Cancer Res. 2005 Aug 15;11(16):6040-9. doi: 10.1158/1078-0432.CCR-04-2273. Clin Cancer Res. 2005. PMID: 16115949 - Anti-invasive effects of green tea polyphenol epigallocatechin-3-gallate (EGCG), a natural inhibitor of metallo and serine proteases.
Benelli R, Venè R, Bisacchi D, Garbisa S, Albini A. Benelli R, et al. Biol Chem. 2002 Jan;383(1):101-5. doi: 10.1515/BC.2002.010. Biol Chem. 2002. PMID: 11928805 Review.
Cited by
- Exploring Senolytic and Senomorphic Properties of Medicinal Plants for Anti-Aging Therapies.
Imb M, Véghelyi Z, Maurer M, Kühnel H. Imb M, et al. Int J Mol Sci. 2024 Sep 27;25(19):10419. doi: 10.3390/ijms251910419. Int J Mol Sci. 2024. PMID: 39408750 Free PMC article. - Effects of Hydrogen Peroxide Produced by Catechins on the Aroma of Tea Beverages.
Wang JQ, Gao Y, Long D, Yin JF, Zeng L, Xu YQ, Xu YQ. Wang JQ, et al. Foods. 2022 Apr 27;11(9):1273. doi: 10.3390/foods11091273. Foods. 2022. PMID: 35563996 Free PMC article. - Radiosensitizing effect of ellagic acid on growth of Hepatocellular carcinoma cells: an in vitro study.
Das U, Biswas S, Chattopadhyay S, Chakraborty A, Dey Sharma R, Banerji A, Dey S. Das U, et al. Sci Rep. 2017 Oct 25;7(1):14043. doi: 10.1038/s41598-017-14211-4. Sci Rep. 2017. PMID: 29070894 Free PMC article. - The Antibacterial Activity of Date Syrup Polyphenols against S. aureus and E. coli.
Taleb H, Maddocks SE, Morris RK, Kanekanian AD. Taleb H, et al. Front Microbiol. 2016 Feb 26;7:198. doi: 10.3389/fmicb.2016.00198. eCollection 2016. Front Microbiol. 2016. PMID: 26952177 Free PMC article. - New insights into the mechanisms of polyphenols beyond antioxidant properties; lessons from the green tea polyphenol, epigallocatechin 3-gallate.
Kim HS, Quon MJ, Kim JA. Kim HS, et al. Redox Biol. 2014 Jan 10;2:187-95. doi: 10.1016/j.redox.2013.12.022. eCollection 2014. Redox Biol. 2014. PMID: 24494192 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials