Green fluorescent protein expression and colocalization with calretinin, parvalbumin, and somatostatin in the GAD67-GFP knock-in mouse - PubMed (original) (raw)
. 2003 Dec 1;467(1):60-79.
doi: 10.1002/cne.10905.
Affiliations
- PMID: 14574680
- DOI: 10.1002/cne.10905
Green fluorescent protein expression and colocalization with calretinin, parvalbumin, and somatostatin in the GAD67-GFP knock-in mouse
Nobuaki Tamamaki et al. J Comp Neurol. 2003.
Abstract
Gamma-aminobutyric acid (GABA)ergic neurons in the central nervous system regulate the activity of other neurons and play a crucial role in information processing. To assist an advance in the research of GABAergic neurons, here we produced two lines of glutamic acid decarboxylase-green fluorescence protein (GAD67-GFP) knock-in mouse. The distribution pattern of GFP-positive somata was the same as that of the GAD67 in situ hybridization signal in the central nervous system. We encountered neither any apparent ectopic GFP expression in GAD67-negative cells nor any apparent lack of GFP expression in GAD67-positive neurons in the two GAD67-GFP knock-in mouse lines. The timing of GFP expression also paralleled that of GAD67 expression. Hence, we constructed a map of GFP distribution in the knock-in mouse brain. Moreover, we used the knock-in mice to investigate the colocalization of GFP with NeuN, calretinin (CR), parvalbumin (PV), and somatostatin (SS) in the frontal motor cortex. The proportion of GFP-positive cells among NeuN-positive cells (neocortical neurons) was approximately 19.5%. All the CR-, PV-, and SS-positive cells appeared positive for GFP. The CR-, PV, and SS-positive cells emitted GFP fluorescence at various intensities characteristics to them. The proportions of CR-, PV-, and SS-positive cells among GFP-positive cells were 13.9%, 40.1%, and 23.4%, respectively. Thus, the three subtypes of GABAergic neurons accounted for 77.4% of the GFP-positive cells. They accounted for 6.5% in layer I. In accord with unidentified GFP-positive cells, many medium-sized spherical somata emitting intense GFP fluorescence were observed in layer I.
Copyright 2003 Wiley-Liss, Inc.
Similar articles
- Quantification and characterization of GABA-ergic amacrine cells in the retina of GAD67-GFP knock-in mice.
May CA, Nakamura K, Fujiyama F, Yanagawa Y. May CA, et al. Acta Ophthalmol. 2008 Jun;86(4):395-400. doi: 10.1111/j.1600-0420.2007.01054.x. Epub 2007 Nov 7. Acta Ophthalmol. 2008. PMID: 17995983 - Fluorescent labeling of both GABAergic and glycinergic neurons in vesicular GABA transporter (VGAT)-venus transgenic mouse.
Wang Y, Kakizaki T, Sakagami H, Saito K, Ebihara S, Kato M, Hirabayashi M, Saito Y, Furuya N, Yanagawa Y. Wang Y, et al. Neuroscience. 2009 Dec 15;164(3):1031-43. doi: 10.1016/j.neuroscience.2009.09.010. Epub 2009 Sep 17. Neuroscience. 2009. PMID: 19766173 - Epithelial localization of green fluorescent protein-positive cells in epididymis of the GAD67-GFP knock-in mouse.
Abe H, Yanagawa Y, Kanbara K, Maemura K, Hayasaki H, Azuma H, Obata K, Katsuoka Y, Yabumoto M, Watanabe M. Abe H, et al. J Androl. 2005 Sep-Oct;26(5):568-77. doi: 10.2164/jandrol.04157. J Androl. 2005. PMID: 16088032 - [GABAergic neurons revealed in the gene knock-out and knock-in mice].
Obata K. Obata K. Tanpakushitsu Kakusan Koso. 2004 Feb;49(3 Suppl):295-300. Tanpakushitsu Kakusan Koso. 2004. PMID: 14976745 Review. Japanese. No abstract available. - Fluorescent in situ hybridization technique for cell type identification and characterization in the central nervous system.
Watakabe A, Komatsu Y, Ohsawa S, Yamamori T. Watakabe A, et al. Methods. 2010 Dec;52(4):367-74. doi: 10.1016/j.ymeth.2010.07.003. Epub 2010 Jul 14. Methods. 2010. PMID: 20637287 Review.
Cited by
- Concerted transcriptional regulation of the morphogenesis of hypothalamic neurons by ONECUT3.
Zupančič M, Keimpema E, Tretiakov EO, Eder SJ, Lev I, Englmaier L, Bhandari P, Fietz SA, Härtig W, Renaux E, Villunger A, Hökfelt T, Zimmer M, Clotman F, Harkany T. Zupančič M, et al. Nat Commun. 2024 Oct 5;15(1):8631. doi: 10.1038/s41467-024-52762-z. Nat Commun. 2024. PMID: 39366958 Free PMC article. - Structural diversity inside the mouse subiculum revealed by a new marker protein fibronectin 1.
Ishihara Y, Miyamoto Y, Esumi S, Fukuda T. Ishihara Y, et al. Anat Sci Int. 2024 Oct 4. doi: 10.1007/s12565-024-00803-4. Online ahead of print. Anat Sci Int. 2024. PMID: 39365413 - Developmental emergence of first- and higher-order thalamic neuron molecular identities.
Lo Giudice Q, Wagener RJ, Abe P, Frangeul L, Jabaudon D. Lo Giudice Q, et al. Development. 2024 Sep 15;151(18):dev202764. doi: 10.1242/dev.202764. Epub 2024 Sep 30. Development. 2024. PMID: 39348458 Free PMC article. - The role of GABA in modulation of taste signaling within the taste bud.
Mikami A, Huang H, Hyodo A, Horie K, Yasumatsu K, Ninomiya Y, Mitoh Y, Iida S, Yoshida R. Mikami A, et al. Pflugers Arch. 2024 Nov;476(11):1761-1775. doi: 10.1007/s00424-024-03007-x. Epub 2024 Aug 29. Pflugers Arch. 2024. PMID: 39210062 Free PMC article. - Stress-induced anxiety-related behavior in mice is driven by enhanced excitability of ventral tegmental area GABA neurons.
Mitten EH, Souders A, Marron Fernandez de Velasco E, Wickman K. Mitten EH, et al. Front Behav Neurosci. 2024 Jul 17;18:1425607. doi: 10.3389/fnbeh.2024.1425607. eCollection 2024. Front Behav Neurosci. 2024. PMID: 39086371 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous