Quantitative metabolome analysis using capillary electrophoresis mass spectrometry - PubMed (original) (raw)
. 2003 Sep-Oct;2(5):488-94.
doi: 10.1021/pr034020m.
Affiliations
- PMID: 14582645
- DOI: 10.1021/pr034020m
Quantitative metabolome analysis using capillary electrophoresis mass spectrometry
Tomoyoshi Soga et al. J Proteome Res. 2003 Sep-Oct.
Abstract
A new approach for the comprehensive and quantitative analysis of charged metabolites by capillary electrophoresis mass spectrometry (CE-MS) is proposed. Metabolites are first separated by CE based on charge and size and then selectively detected using MS by monitoring over a large range of m/z values. This method enabled the determination of 352 metabolic standards and its utility was demonstrated in the analysis of 1692 metabolites from Bacillus subtilis extracts, revealing significant changes in metabolites during B. subtilis sporulation.
Comment in
- CE/MS for quantitative metabolome analysis.
Smith WD. Smith WD. J Proteome Res. 2003 Sep-Oct;2(5):463. doi: 10.1021/pr0307467. J Proteome Res. 2003. PMID: 14582642 No abstract available.