Total body protein: a new cellular level mass and distribution prediction model - PubMed (original) (raw)

Comparative Study

Total body protein: a new cellular level mass and distribution prediction model

ZiMian Wang et al. Am J Clin Nutr. 2003 Nov.

Abstract

Background: Protein is an important body component, and the presently accepted criterion method for estimating total body protein (TBPro) mass--in vivo neutron activation (IVNA) analysis--is unavailable to most investigators and is associated with moderate radiation exposure.

Objective: The objective was to derive a theoretical cellular level TBPro mass and distribution model formulated on measured total body potassium, total body water, and bone mineral and to evaluate the new model with the IVNA method as the criterion.

Design: The new model was developed on the basis of a combination of theoretical equations and empirically derived coefficients. TBPro mass estimates with the new model were evaluated in healthy women (n = 183) and men (n = 24) and in men with AIDS (n = 84). Total body nitrogen was measured by IVNA, total body potassium by whole-body (40)K counting, total body water by tritium dilution, and bone mineral by dual-energy X-ray absorptiometry.

Results: The group mean (+/- SD) TBPro mass estimates in healthy women and men and men with AIDS (8.2 +/- 0.9, 11.0 +/- 1.8, and 10.5 +/- 1.1 kg, respectively) with the new model were similar to IVNA criterion estimates (8.9 +/- 0.9, 11.1 +/- 1.6, and 10.9 +/- 1.2 kg, respectively). TBPro mass estimates with the new model correlated highly with the IVNA estimates in all subjects combined (r = 0.92, P < 0.001). The new model suggests that the composite TBPro mass within each group consists mainly of cellular protein (75-79%) and, to a lesser extent, protein in extracellular solids (19-23%) and extracellular fluid (approximately 2%).

Conclusion: The new model provides a non-IVNA approach for estimating protein mass and distribution in vivo.

PubMed Disclaimer

Cited by

Proteomic data from human cell cultures refine mechanisms of chaperone-mediated protein homeostasis.
Finka A, Goloubinoff P. Finka A, et al. Cell Stress Chaperones. 2013 Sep;18(5):591-605. doi: 10.1007/s12192-013-0413-3. Epub 2013 Feb 21. Cell Stress Chaperones. 2013. PMID: 23430704 Free PMC article.
Nutritional Status and Body Composition at Diagnosis, of South Indian Children with Acute Lymphoblastic Leukaemia (ALL).
Puttaswamy D, Ramesh V, Raj T, Bhat KG, Selvam S, Bharadwaj V, Prakash A, Murphy-Alford AJ, Kuriyan R. Puttaswamy D, et al. Asian Pac J Cancer Prev. 2024 Jul 1;25(7):2361-2369. doi: 10.31557/APJCP.2024.25.7.2361. Asian Pac J Cancer Prev. 2024. PMID: 39068569 Free PMC article.
A new total body potassium method to estimate total body skeletal muscle mass in children.
Wang Z, Heshka S, Pietrobelli A, Chen Z, Silva AM, Sardinha LB, Wang J, Gallager D, Heymsfield SB. Wang Z, et al. J Nutr. 2007 Aug;137(8):1988-91. doi: 10.1093/jn/137.8.1988. J Nutr. 2007. PMID: 17634275 Free PMC article.
Proline metabolism and microenvironmental stress.
Phang JM, Liu W, Zabirnyk O. Phang JM, et al. Annu Rev Nutr. 2010 Aug 21;30:441-63. doi: 10.1146/annurev.nutr.012809.104638. Annu Rev Nutr. 2010. PMID: 20415579 Free PMC article. Review.
Amino acid-induced regulation of hepatocyte growth: possible role of Drosha.
Fabris G, Dumortier O, Pisani DF, Gautier N, Van Obberghen E. Fabris G, et al. Cell Death Dis. 2019 Jul 22;10(8):566. doi: 10.1038/s41419-019-1779-7. Cell Death Dis. 2019. PMID: 31332188 Free PMC article.

Total body protein: a new cellular level mass and distribution prediction model - PubMed (original) (raw)