Determination of peptides and proteins in human urine with capillary electrophoresis-mass spectrometry, a suitable tool for the establishment of new diagnostic markers - PubMed (original) (raw)
. 2003 Sep 26;1013(1-2):173-81.
doi: 10.1016/s0021-9673(03)00713-1.
Danilo Fliser, Marion Haubitz, Sebastian Bartel, Ronald Krebs, Frank Hausadel, Meike Hillmann, Igor Golovko, Peer Koester, Hermann Haller, Thorsten Kaiser, Harald Mischak, Eva M Weissinger
Affiliations
- PMID: 14604118
- DOI: 10.1016/s0021-9673(03)00713-1
Determination of peptides and proteins in human urine with capillary electrophoresis-mass spectrometry, a suitable tool for the establishment of new diagnostic markers
Stefan Wittke et al. J Chromatogr A. 2003.
Abstract
The on-line coupling of capillary electrophoresis (CE) with electrospray-time-of-flight mass spectrometry (MS) has been used to obtain patterns of peptides and proteins present in the urine of healthy human individuals. This led to the establishment of a "normal urine polypeptide pattern", consisting of 247 polypeptides, each of which was found in more than 50% of healthy individuals. Applying CE-MS to the analysis of urine of patients with kidney disease revealed differences in polypeptide pattern. Twenty-seven polypeptides were exclusively found in samples of patients. Another 13, present in controls, were missing. These data indicate that CE-MS can be applied as powerful tool in clinical diagnostics.
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