Molecular surveillance of Cryptosporidium spp. in raw wastewater in Milwaukee: implications for understanding outbreak occurrence and transmission dynamics - PubMed (original) (raw)
Molecular surveillance of Cryptosporidium spp. in raw wastewater in Milwaukee: implications for understanding outbreak occurrence and transmission dynamics
Ling Zhou et al. J Clin Microbiol. 2003 Nov.
Erratum in
- J Clin Microbiol. 2004 Apr;42(4):1859
Abstract
Six Cryptosporidium spp. were found in 50 of 179 Milwaukee wastewater samples collected weekly over a year. Of the eight subtypes of Cryptosporidium hominis and Cryptosporidium parvum present, allele Ib was found in 14 of 16 samples, and its sequence was identical to that of the subtype in human samples from the 1993 Milwaukee outbreak of cryptosporidiosis.
Figures
FIG. 1.
Relationship among C. parvum and C. hominis subtypes inferred by neighbor-joining analysis of GP60 nucleotide sequences. The Kimura two-parameter model was used in distance calculation. Numbers on branches are percent bootstrap values from 1,000 resamplings.
References
- Leav, B. A., M. R. Mackay, A. Anyanwu, R. M. O'Connor, A. M. Cevallos, G. Kindra, N. C. Rollins, M. L. Bennish, R. G. Nelson, and H. D. Ward. 2002. Analysis of sequence diversity at the highly polymorphic Cpgp40/15 locus among Cryptosporidium isolates from human immunodeficiency virus-infected children in South Africa. Infect. Immun. 70**:**3881-3890. - PMC - PubMed
- McLauchlin, J., C. Amar, S. Pedraza-Diaz, and G. L. J. Nichols. 2000. Molecular epidemiological analysis of Cryptosporidium spp. in the United Kingdom: results of genotyping Cryptosporidium spp. in 1,705 fecal samples from humans and 105 fecal samples from livestock animals. J. Clin. Microbiol. 38**:**3984-3990. - PMC - PubMed
- Peng, M. M., O. Matos, W. Gatei, P. Das, M. Stantic-Pavlinic, C. Bern, I. M. Sulaiman, S. Glaberman, A. A. Lal, and L. Xiao. 2001. A comparison of Cryptosporidium subgenotypes from several geographic regions. J. Eukaryot. Microbiol. 2001(Suppl.)**:**28S-31S. - PubMed
- Peng, M. M., L. Xiao, A. R. Freeman, M. J. Arrowood, A. A. Escalante, A. C. Weltman, C. S. Ong, W. R. MacKenzie, A. A. Lal, and C. B. Beard. 1997. Genetic polymorphism among Cryptosporidium parvum isolates: evidence of two distinct human transmission cycles. Emerg. Infect. Dis. 3**:**567-573. - PMC - PubMed
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