Expression of B-cell markers in classical hodgkin lymphoma: a tissue microarray analysis of 330 cases - PubMed (original) (raw)
Expression of B-cell markers in classical hodgkin lymphoma: a tissue microarray analysis of 330 cases
Alexandar Tzankov et al. Mod Pathol. 2003 Nov.
Abstract
Hodgkin and Reed-Sternberg cells of classical Hodgkin lymphoma arise from B-lymphocytes. However, classical markers of the B-cell phenotype, such as CD20, are present only in about 25% of the cases. The aim of the present study was to assess expression of the B-cell-related antigens CD20, CD79a, and CD138 in classical Hodgkin lymphoma using a tissue microarray consisting of 330 classical Hodgkin lymphoma cases. Expression of CD15, CD20, CD30, CD79a, CD138, and latent membrane protein 1 of Epstein-Barr virus was assessed by immunohistochemistry, and the methodology was validated by direct comparison of CD20 expression on the tissue microarray cores with corresponding large sections. The influence of the number of arrayed sample cores on the obtained expression levels of CD20 was analyzed by comparing the results from single, duplicate, and triplicate cores. Two-hundred fifty-three (77%) of the 330 cases were morphologically representative. CD20 was expressed in 84 cases (33%), CD79a in 26 (10%), and CD138 in 2 (1%), respectively. CD20 and CD79a were co-expressed in 16 cases (P <.005), and expression of CD20 correlated inversely with CD15 (P <.01). Comparing the tissue microarray results with those from conventional sections for expression of CD20 yielded a concordance of 94% (63/67). Examining one, two, and three cores from individual cases revealed positivity for CD20 at 24% (61/253), 32% (82/253), and 33% (84/253), respectively. We conclude that B-cell markers are expressed in 38% of classical Hodgkin lymphoma in the following rank order: CD20>CD79a>>CD138. The use of two cores per tissue sample renders the tissue microarray technology effectively representative and thus very useful for high-throughput evaluation of heterogeneously expressed markers in classical Hodgkin lymphoma.
Similar articles
- Composite marginal zone B-cell lymphoma and classical Hodgkin's lymphoma: a clinicopathological study of 12 cases.
Zettl A, Rüdiger T, Marx A, Müller-Hermelink HK, Ott G. Zettl A, et al. Histopathology. 2005 Feb;46(2):217-28. doi: 10.1111/j.1365-2559.2005.02046.x. Histopathology. 2005. PMID: 15693895 - Evidence of abortive plasma cell differentiation in Hodgkin and Reed-Sternberg cells of classical Hodgkin lymphoma.
Buettner M, Greiner A, Avramidou A, Jäck HM, Niedobitek G. Buettner M, et al. Hematol Oncol. 2005 Sep-Dec;23(3-4):127-32. doi: 10.1002/hon.764. Hematol Oncol. 2005. PMID: 16342298 Review. - Composite recurrent hodgkin lymphoma and diffuse large B-cell lymphoma: one clone, two faces.
Huang Q, Wilczynski SP, Chang KL, Weiss LM. Huang Q, et al. Am J Clin Pathol. 2006 Aug;126(2):222-9. doi: 10.1309/DDGL-WRV3-KR91-64G7. Am J Clin Pathol. 2006. PMID: 16891197 - BOB.1, CD79a and cyclin E are the most appropriate markers to discriminate classical Hodgkin's lymphoma from primary mediastinal large B-cell lymphoma.
Hoeller S, Zihler D, Zlobec I, Obermann EC, Pileri SA, Dirnhofer S, Tzankov A. Hoeller S, et al. Histopathology. 2010 Jan;56(2):217-28. doi: 10.1111/j.1365-2559.2009.03462.x. Histopathology. 2010. PMID: 20102401 - [Molecular biology aspects of Hodgkin's disease].
Macák J. Macák J. Vnitr Lek. 1998 Jun;44(6):370-2. Vnitr Lek. 1998. PMID: 9820063 Review. Czech.
Cited by
- Primary gastric Hodgkin's lymphoma.
Hossain FS, Koak Y, Khan FH. Hossain FS, et al. World J Surg Oncol. 2007 Oct 21;5:119. doi: 10.1186/1477-7819-5-119. World J Surg Oncol. 2007. PMID: 17949508 Free PMC article. - Hodgkin lymphoma: an update on its biology with new insights into classification.
Mani H, Jaffe ES. Mani H, et al. Clin Lymphoma Myeloma. 2009 Jun;9(3):206-16. doi: 10.3816/CLM.2009.n.042. Clin Lymphoma Myeloma. 2009. PMID: 19525189 Free PMC article. Review. - Tissue microarray technique is applicable to bone marrow biopsies of myeloproliferative neoplasms.
Limberger KA, Bogatyreva L, Todorova R, Herde B, Hauschke D, Pahl HL, Werner M, Aumann K. Limberger KA, et al. Histochem Cell Biol. 2017 Jan;147(1):75-82. doi: 10.1007/s00418-016-1476-x. Epub 2016 Aug 20. Histochem Cell Biol. 2017. PMID: 27544663 - Fine-tuning of FOXO3A in cHL as a survival mechanism and a hallmark of abortive plasma cell differentiation.
Osswald CD, Xie L, Guan H, Herrmann F, Pick SM, Vogel MJ, Gehringer F, Chan FC, Steidl C, Wirth T, Ushmorov A. Osswald CD, et al. Blood. 2018 Apr 5;131(14):1556-1567. doi: 10.1182/blood-2017-07-795278. Epub 2018 Feb 13. Blood. 2018. PMID: 29439954 Free PMC article. - Pathobiology of hodgkin lymphoma.
Agostinelli C, Pileri S. Agostinelli C, et al. Mediterr J Hematol Infect Dis. 2014 Jun 5;6(1):e2014040. doi: 10.4084/MJHID.2014.040. eCollection 2014. Mediterr J Hematol Infect Dis. 2014. PMID: 24959337 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Research Materials
Miscellaneous