Characterization of activated and normal mouse Mos gene in murine 3T3 cells - PubMed (original) (raw)
. 1992 Dec;7(12):2489-98.
Affiliations
- PMID: 1461652
Characterization of activated and normal mouse Mos gene in murine 3T3 cells
R S Paules et al. Oncogene. 1992 Dec.
Abstract
We have characterized the mouse Mos proto-oncogene product, pp39Mos, in murine fibroblasts. When expressed in NIH3T3 cells under the influence of the long terminal repeat regulatory element from Moloney murine sarcoma virus [NIH(pTS-1) cells], the Mos protein was present in low levels and had a half-life of about 30 min. In extracts from NIH(pTS-1) cells, we detected additional forms of Mos protein that apparently arose from internal initiation codons (p24Mos and p29Mos) or from upstream non-AUG initiation codons (p42Mos and p44Mos). The Mos protein was found to exist in these cells as a phosphoprotein, pp39Mos, and, when immunoprecipitated with an antiserum specific for the Mos N-terminus [anti-Mos(6-24)], had autophosphorylating kinase activity. We found that anti-Mos(6-24) also detected non-Mos protein kinase activity and non-Mos phosphoproteins in addition to p39Mos. We present evidence, on both the RNA and protein levels, that non-transformed mouse 3T3 cells do not express endogenous Mos.
Similar articles
- Oncogenic activation of murine mos protein kinase by DNA rearrangement of its N-terminal coding region.
Ohuchi T, Kurita Y, Sasai H, Miyoshi J, Nomura T, Toyoshima K. Ohuchi T, et al. Oncogene. 1992 Feb;7(2):331-8. Oncogene. 1992. PMID: 1532243 - Further characterization of the c-mos transcript and its cell cycle specific expression in NIH3T3 cells.
Gao C, Arlinghaus RB, Singh B. Gao C, et al. Oncogene. 1996 Apr 4;12(7):1571-6. Oncogene. 1996. PMID: 8622874 - Functions of the mos oncogene family and associated gene products.
Bold RJ, Hannink M, Donoghue DJ. Bold RJ, et al. Cancer Surv. 1986;5(2):243-55. Cancer Surv. 1986. PMID: 2946405 - Overproduction of metalloproteinases by v-mos-transformed rat kidney (6m2) cells.
Chan JC. Chan JC. Leukemia. 1992;6 Suppl 3:168S-170S. Leukemia. 1992. PMID: 1602817 Review.
Cited by
- Signaling pathways in Ras-mediated tumorigenicity and metastasis.
Webb CP, Van Aelst L, Wigler MH, Vande Woude GF. Webb CP, et al. Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):8773-8. doi: 10.1073/pnas.95.15.8773. Proc Natl Acad Sci U S A. 1998. PMID: 9671754 Free PMC article. - Identification of a candidate c-mos repressor that restricts transcription of germ cell-specific genes.
Xu W, Cooper GM. Xu W, et al. Mol Cell Biol. 1995 Oct;15(10):5369-75. doi: 10.1128/MCB.15.10.5369. Mol Cell Biol. 1995. PMID: 7565687 Free PMC article. - Membrane localization of phosphatidylinositol 3-kinase is sufficient to activate multiple signal-transducing kinase pathways.
Klippel A, Reinhard C, Kavanaugh WM, Apell G, Escobedo MA, Williams LT. Klippel A, et al. Mol Cell Biol. 1996 Aug;16(8):4117-27. doi: 10.1128/MCB.16.8.4117. Mol Cell Biol. 1996. PMID: 8754810 Free PMC article. - ERF: an ETS domain protein with strong transcriptional repressor activity, can suppress ets-associated tumorigenesis and is regulated by phosphorylation during cell cycle and mitogenic stimulation.
Sgouras DN, Athanasiou MA, Beal GJ Jr, Fisher RJ, Blair DG, Mavrothalassitis GJ. Sgouras DN, et al. EMBO J. 1995 Oct 2;14(19):4781-93. doi: 10.1002/j.1460-2075.1995.tb00160.x. EMBO J. 1995. PMID: 7588608 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Molecular Biology Databases