The spliceosome: the most complex macromolecular machine in the cell? - PubMed (original) (raw)
Review
. 2003 Dec;25(12):1147-9.
doi: 10.1002/bies.10394.
Affiliations
- PMID: 14635248
- DOI: 10.1002/bies.10394
Review
The spliceosome: the most complex macromolecular machine in the cell?
Timothy W Nilsen. Bioessays. 2003 Dec.
Abstract
The primary transcripts, pre-mRNAs, of almost all protein-coding genes in higher eukaryotes contain multiple non-coding intervening sequences, introns, which must be precisely removed to yield translatable mRNAs. The process of intron excision, splicing, takes place in a massive ribonucleoprotein complex known as the spliceosome. Extensive studies, both genetic and biochemical, in a variety of systems have revealed that essential components of the spliceosome include five small RNAs-U1, U2, U4, U5 and U6, each of which functions as a RNA, protein complex called an snRNP (small nuclear ribonucleoprotein). In addition to snRNPs, splicing requires many non-snRNP protein factors, the exact nature and number of which has been unclear. Technical advances, including new affinity purification methods and improved mass spectrometry techniques, coupled with the completion of many genome sequences, have now permitted a number of proteomic analyses of purified spliceosomes. These studies, recently reviewed by Jurica and Moore,1 reveal that the spliceosome is composed of as many as 300 distinct proteins and five RNAs, making it among the most complex macromolecular machines known.
Copyright 2003 Wiley Periodicals, Inc.
Similar articles
- The spliceosome.
Lamond AI. Lamond AI. Bioessays. 1993 Sep;15(9):595-603. doi: 10.1002/bies.950150905. Bioessays. 1993. PMID: 8240312 Review. - Roles of the U5 snRNP in spliceosome dynamics and catalysis.
Turner IA, Norman CM, Churcher MJ, Newman AJ. Turner IA, et al. Biochem Soc Trans. 2004 Dec;32(Pt 6):928-31. doi: 10.1042/BST0320928. Biochem Soc Trans. 2004. PMID: 15506927 Review. - A novel yeast U2 snRNP protein, Snu17p, is required for the first catalytic step of splicing and for progression of spliceosome assembly.
Gottschalk A, Bartels C, Neubauer G, Lührmann R, Fabrizio P. Gottschalk A, et al. Mol Cell Biol. 2001 May;21(9):3037-46. doi: 10.1128/MCB.21.9.3037-3046.2001. Mol Cell Biol. 2001. PMID: 11287609 Free PMC article. - Splicing-independent recruitment of spliceosomal small nuclear RNPs to nascent RNA polymerase II transcripts.
Patel SB, Novikova N, Bellini M. Patel SB, et al. J Cell Biol. 2007 Sep 10;178(6):937-49. doi: 10.1083/jcb.200706134. J Cell Biol. 2007. PMID: 17846169 Free PMC article. - Small nuclear ribonucleoprotein remodeling during catalytic activation of the spliceosome.
Makarov EM, Makarova OV, Urlaub H, Gentzel M, Will CL, Wilm M, Lührmann R. Makarov EM, et al. Science. 2002 Dec 13;298(5601):2205-8. doi: 10.1126/science.1077783. Epub 2002 Oct 31. Science. 2002. PMID: 12411573
Cited by
- Study of the RNA splicing kinetics via in vivo 5-EU labeling.
Bolikhova AK, Buyan AI, Mariasina SS, Rudenko AY, Chekh DS, Mazur AM, Prokhortchouk EB, Dontsova OA, Sergiev PV. Bolikhova AK, et al. RNA. 2024 Sep 16;30(10):1356-1373. doi: 10.1261/rna.079937.123. RNA. 2024. PMID: 39048310 - Introns and Their Therapeutic Applications in Biomedical Researches.
Haddad-Mashadrizeh A, Mirahmadi M, Taghavizadeh Yazdi ME, Gholampour-Faroji N, Bahrami A, Zomorodipour A, Moghadam Matin M, Qayoomian M, Saebnia N. Haddad-Mashadrizeh A, et al. Iran J Biotechnol. 2023 Oct 1;21(4):e3316. doi: 10.30498/ijb.2023.334488.3316. eCollection 2023 Oct. Iran J Biotechnol. 2023. PMID: 38269198 Free PMC article. Review. - Morphology, Histology, and Transcriptome Analysis of Gonadal Development in Octopus minor (Sasaki, 1920).
Li J, Zheng X. Li J, et al. Mar Biotechnol (NY). 2023 Dec;25(6):1043-1056. doi: 10.1007/s10126-023-10258-9. Epub 2023 Oct 25. Mar Biotechnol (NY). 2023. PMID: 37878213 - Identification of pararosaniline as a modifier of RNA splicing in Caenorhabditis elegans.
Huynh D, Wu CW. Huynh D, et al. G3 (Bethesda). 2023 Dec 6;13(12):jkad241. doi: 10.1093/g3journal/jkad241. G3 (Bethesda). 2023. PMID: 37852248 Free PMC article. - Conserved and divergent signals in 5' splice site sequences across fungi, metazoa and plants.
Beckel MS, Kaufman B, Yanovsky M, Chernomoretz A. Beckel MS, et al. PLoS Comput Biol. 2023 Oct 13;19(10):e1011540. doi: 10.1371/journal.pcbi.1011540. eCollection 2023 Oct. PLoS Comput Biol. 2023. PMID: 37831726 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources