Sps-3 transcript levels are determined by multiple remote sequence elements - PubMed (original) (raw)
Sps-3 transcript levels are determined by multiple remote sequence elements
A Giangrande et al. EMBO J. 1987 Oct.
Abstract
The region from 1.4 to 2.7 kb upstream of Drosophila melanogaster gene Sgs-3 is responsible for a 10-fold increase in Sgs-3 transcript levels in the third instar larval salivary gland. This region includes the related Sgs-7 gene from the 68C glue gene cluster as well as 400 bp of its 5' sequences. We show that two elements are involved, each contributing a modest 3-fold effect. One of these includes Sgs-7 transcribed sequences some 2.3 kb upstream of Sgs-3, although Sgs-7 transcription is not involved. Although important for the overall levels of Sgs-3 expression, they are clearly not strong, viral-like enhancer elements. We propose that many position effects observed in P element transformation studies are the consequence of insertion in the vicinity of similar elements dispersed throughout the genome and having modest effects on transcript levels.
References
- Cell. 1984 Aug;38(1):135-46 - PubMed
- EMBO J. 1986 Dec 1;5(12):3321-6 - PubMed
- Cell. 1986 Jun 20;45(6):859-67 - PubMed
- J Mol Biol. 1983 Aug 25;168(4):765-89 - PubMed
- Science. 1986 Nov 21;234(4779):998-1002 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases