Identification of four genes necessary for biosynthesis of the modified nucleoside queuosine - PubMed (original) (raw)
. 2004 Feb 20;279(8):6280-5.
doi: 10.1074/jbc.M310858200. Epub 2003 Dec 2.
Affiliations
- PMID: 14660578
- DOI: 10.1074/jbc.M310858200
Free article
Identification of four genes necessary for biosynthesis of the modified nucleoside queuosine
John S Reader et al. J Biol Chem. 2004.
Free article
Abstract
Queuosine (Q) is a hypermodified 7-deazaguanosine nucleoside located in the anticodon wobble position of four amino acid-specific tRNAs. In bacteria, Q is produced de novo from GTP via the 7-deazaguanosine precursor preQ1 (7-aminoethyl 7-deazaguanine) by an uncharacterized pathway. PreQ1 is subsequently transferred to its specific tRNA by a tRNA-guanine transglycosylase (TGT) and then further modified in situ to produce Q. Here we use comparative genomics to implicate four gene families (best exemplified by the B. subtilis operon ykvJKLM) as candidates in the preQ1 biosynthetic pathway. Deletions were constructed in genes for each of the four orthologs in Acinetobacter. High pressure liquid chromatography analysis showed the Q nucleoside was absent from the tRNAs of each of four deletion strains. Electrospray ionization mass spectrometry confirmed the absence of Q in each mutant strain. Finally, introduction of the Bacillus subtilis ykvJKLM operon in trans complemented the Q deficiency of the two deletion mutants that were tested. Thus, the products of these four genes (named queC, -D, -E, and -F) are essential for the Q biosynthetic pathway.
Similar articles
- The Escherichia coli COG1738 Member YhhQ Is Involved in 7-Cyanodeazaguanine (preQ₀) Transport.
Zallot R, Yuan Y, de Crécy-Lagard V. Zallot R, et al. Biomolecules. 2017 Feb 8;7(1):12. doi: 10.3390/biom7010012. Biomolecules. 2017. PMID: 28208705 Free PMC article. - Biosynthesis of the 7-deazaguanosine hypermodified nucleosides of transfer RNA.
Iwata-Reuyl D. Iwata-Reuyl D. Bioorg Chem. 2003 Feb;31(1):24-43. doi: 10.1016/s0045-2068(02)00513-8. Bioorg Chem. 2003. PMID: 12697167 Review. - Biosynthesis of 7-deazaguanosine-modified tRNA nucleosides: a new role for GTP cyclohydrolase I.
Phillips G, El Yacoubi B, Lyons B, Alvarez S, Iwata-Reuyl D, de Crécy-Lagard V. Phillips G, et al. J Bacteriol. 2008 Dec;190(24):7876-84. doi: 10.1128/JB.00874-08. Epub 2008 Oct 17. J Bacteriol. 2008. PMID: 18931107 Free PMC article. - A new function of S-adenosylmethionine: the ribosyl moiety of AdoMet is the precursor of the cyclopentenediol moiety of the tRNA wobble base queuine.
Slany RK, Bösl M, Crain PF, Kersten H. Slany RK, et al. Biochemistry. 1993 Aug 3;32(30):7811-7. doi: 10.1021/bi00081a028. Biochemistry. 1993. PMID: 8347586 - Genes, enzymes and coenzymes of queuosine biosynthesis in procaryotes.
Slany RK, Kersten H. Slany RK, et al. Biochimie. 1994;76(12):1178-82. doi: 10.1016/0300-9084(94)90047-7. Biochimie. 1994. PMID: 7748953 Review.
Cited by
- Queuosine biosynthetic enzyme, QueE moonlights as a cell division regulator.
Adeleye SA, Yadavalli SS. Adeleye SA, et al. PLoS Genet. 2024 May 20;20(5):e1011287. doi: 10.1371/journal.pgen.1011287. eCollection 2024 May. PLoS Genet. 2024. PMID: 38768229 Free PMC article. - Snow viruses and their implications on red snow algal blooms.
Barno AR, Green K, Rohwer F, Silveira CB. Barno AR, et al. mSystems. 2024 May 16;9(5):e0008324. doi: 10.1128/msystems.00083-24. Epub 2024 Apr 22. mSystems. 2024. PMID: 38647296 Free PMC article. - Alternate routes to mnm5s2U synthesis in Gram-positive bacteria.
Jaroch M, Sun G, Tsui H-CT, Reed C, Sun J, Jörg M, Winkler ME, Rice KC, Dziergowska A, Stich TA, Dedon PC, Dos Santos PC, de Crécy-Lagard V. Jaroch M, et al. J Bacteriol. 2024 Apr 18;206(4):e0045223. doi: 10.1128/jb.00452-23. Epub 2024 Mar 29. J Bacteriol. 2024. PMID: 38551342 Free PMC article. - Biosynthesis and function of 7-deazaguanine derivatives in bacteria and phages.
de Crécy-Lagard V, Hutinet G, Cediel-Becerra JDD, Yuan Y, Zallot R, Chevrette MG, Ratnayake RMMN, Jaroch M, Quaiyum S, Bruner S. de Crécy-Lagard V, et al. Microbiol Mol Biol Rev. 2024 Mar 27;88(1):e0019923. doi: 10.1128/mmbr.00199-23. Epub 2024 Feb 29. Microbiol Mol Biol Rev. 2024. PMID: 38421302 Review. - Detection of queuosine and queuosine precursors in tRNAs by direct RNA sequencing.
Sun Y, Piechotta M, Naarmann-de Vries I, Dieterich C, Ehrenhofer-Murray AE. Sun Y, et al. Nucleic Acids Res. 2023 Nov 10;51(20):11197-11212. doi: 10.1093/nar/gkad826. Nucleic Acids Res. 2023. PMID: 37811872 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases