Near-infrared fluorescent type II quantum dots for sentinel lymph node mapping - PubMed (original) (raw)
doi: 10.1038/nbt920. Epub 2003 Dec 7.
Yong Taik Lim, Edward G Soltesz, Alec M De Grand, Jaihyoung Lee, Akira Nakayama, J Anthony Parker, Tomislav Mihaljevic, Rita G Laurence, Delphine M Dor, Lawrence H Cohn, Moungi G Bawendi, John V Frangioni
Affiliations
- PMID: 14661026
- PMCID: PMC2346610
- DOI: 10.1038/nbt920
Near-infrared fluorescent type II quantum dots for sentinel lymph node mapping
Sungjee Kim et al. Nat Biotechnol. 2004 Jan.
Abstract
The use of near-infrared or infrared photons is a promising approach for biomedical imaging in living tissue. This technology often requires exogenous contrast agents with combinations of hydrodynamic diameter, absorption, quantum yield and stability that are not possible with conventional organic fluorophores. Here we show that the fluorescence emission of type II quantum dots can be tuned into the near infrared while preserving absorption cross-section, and that a polydentate phosphine coating renders them soluble, disperse and stable in serum. We then demonstrate that these quantum dots allow a major cancer surgery, sentinel lymph node mapping, to be performed in large animals under complete image guidance. Injection of only 400 pmol of near-infrared quantum dots permits sentinel lymph nodes 1 cm deep to be imaged easily in real time using excitation fluence rates of only 5 mW/cm(2). Taken together, the chemical, optical and in vivo data presented in this study demonstrate the potential of near-infrared quantum dots for biomedical imaging.
Figures
Figure 1
Physical and optical properties of aqueous-soluble, NIR type II QDs. (a) TEM image of water-dispersed NIR QDs. (b) Molar extinction coefficient (solid curve; left axis) and photoluminescence intensity (dashed curve; right axis) of NIR QDs in PBS, pH 7.4. (c) Gel-filtration chromatography of protein standards (left) and NIR QDs (right) in PBS, pH 7.4. Standards included thyroglobulin (669 kDa; solid curve), alcohol dehydrogenase (150 kDa; thick solid curve) and ovalbumin (44 kDa; dotted curve). NIR QDs had an effective molecular weight of 440 kDa. (d) Fluorescence stability of 1 μM organic NIR fluorophore IRDye78-CA (left) and 1 μM NIR QDs (right), in 100% FCS, as a function of excitation fluence rate and time. Note that the abscissa for IRDye78-CA is in seconds and that for NIR QDs is in minutes. (e) Fluorescence stability of 1 μM NIR QDs, in 100% FCS, at 37 °C over time.
Figure 2
NIR QD sentinel lymph node mapping in the mouse and pig. (a) Images of mouse injected intradermally with 10 pmol of NIR QDs in the left paw. Left, pre-injection NIR autofluorescence image; middle, 5 min post-injection white light color video image; right, 5 min post-injection NIR fluorescence image. An arrow indicates the putative axillary sentinel lymph node. Fluorescence images have identical exposure times and normalization. (b) Images of the mouse shown in a 5 min after reinjection with 1% isosulfan blue and exposure of the actual sentinel lymph node. Left, color video; right, NIR fluorescence images. Isosulfan blue and NIR QDs were localized in the same lymph node (arrows). (c) Images of the surgical field in a pig injected intradermally with 400 pmol of NIR QDs in the right groin. Four time points are shown from top to bottom: before injection (autofluorescence), 30 s after injection, 4 min after injection and during image-guided resection. For each time point, color video (left), NIR fluorescence (middle) and color-NIR merge (right) images are shown. Fluorescence images have identical exposure times and normalization. To create the merged image, the NIR fluorescence image was pseudocolored lime green and superimposed on the color video image. The position of a nipple (N) is indicated.
Figure 3
Post-resection inspection of the surgical field and evaluation of NIR QD lymph node retention. (a) Post-resection evaluation of the surgical field. Shown are color video (left), NIR fluorescence (middle) and color-NIR merge (right) images. Arrows indicate the resected sentinel lymph node. (b) NIR QD retention by the resected SLN (S) and the next lymph node (N) in the chain is shown in this bisected specimen. (c) Histologic analysis of frozen sections of the SLN in b. Shown are two representative hematoxylin and eosin (H + E)-stained sections and consecutive unstained sections photographed on a NIR fluorescence microscope.
Comment in
- Cancer surgery joins the dots.
Uren RF. Uren RF. Nat Biotechnol. 2004 Jan;22(1):38-9. doi: 10.1038/nbt0104-38. Nat Biotechnol. 2004. PMID: 14704701 No abstract available.
Similar articles
- Intraoperative sentinel lymph node mapping of the lung using near-infrared fluorescent quantum dots.
Soltesz EG, Kim S, Laurence RG, DeGrand AM, Parungo CP, Dor DM, Cohn LH, Bawendi MG, Frangioni JV, Mihaljevic T. Soltesz EG, et al. Ann Thorac Surg. 2005 Jan;79(1):269-77; discussion 269-77. doi: 10.1016/j.athoracsur.2004.06.055. Ann Thorac Surg. 2005. PMID: 15620956 Free PMC article. - Image-guided oncologic surgery using invisible light: completed pre-clinical development for sentinel lymph node mapping.
Tanaka E, Choi HS, Fujii H, Bawendi MG, Frangioni JV. Tanaka E, et al. Ann Surg Oncol. 2006 Dec;13(12):1671-81. doi: 10.1245/s10434-006-9194-6. Epub 2006 Sep 29. Ann Surg Oncol. 2006. PMID: 17009138 Free PMC article. - Sentinel lymph node mapping of the gastrointestinal tract by using invisible light.
Soltesz EG, Kim S, Kim SW, Laurence RG, De Grand AM, Parungo CP, Cohn LH, Bawendi MG, Frangioni JV. Soltesz EG, et al. Ann Surg Oncol. 2006 Mar;13(3):386-96. doi: 10.1245/ASO.2006.04.025. Epub 2006 Jan 31. Ann Surg Oncol. 2006. PMID: 16485157 - Image-guided sentinel lymph node mapping and nanotechnology-based nodal treatment in lung cancer using invisible near-infrared fluorescent light.
Khullar O, Frangioni JV, Grinstaff M, Colson YL. Khullar O, et al. Semin Thorac Cardiovasc Surg. 2009 Winter;21(4):309-15. doi: 10.1053/j.semtcvs.2009.11.009. Semin Thorac Cardiovasc Surg. 2009. PMID: 20226343 Free PMC article. Review. - Hybrid tracers for sentinel node biopsy.
Van Den Berg NS, Buckle T, Kleinjan GI, Klop WM, Horenblas S, Van Der Poel HG, Valdés-Olmos RA, Van Leeuwen FI. Van Den Berg NS, et al. Q J Nucl Med Mol Imaging. 2014 Jun;58(2):193-206. Q J Nucl Med Mol Imaging. 2014. PMID: 24835293 Review.
Cited by
- 2D compounds with heterolayered architecture for infrared photodetectors.
Gu H, Zhang T, Wang Y, Zhou T, Chen H. Gu H, et al. Chem Sci. 2024 Sep 9;15(39):15983-6005. doi: 10.1039/d4sc03428g. Online ahead of print. Chem Sci. 2024. PMID: 39328196 Free PMC article. Review. - Protocol for quantum dot-based cell counting and immunostaining of pulmonary arterial cells from patients with pulmonary arterial hypertension.
Moinuddin SM, Ibrahim M, Sarkar T, Hossain MS, Rose M, Ahsan F. Moinuddin SM, et al. STAR Protoc. 2024 Sep 18;5(4):103319. doi: 10.1016/j.xpro.2024.103319. Online ahead of print. STAR Protoc. 2024. PMID: 39298320 Free PMC article. - Exploring the full potential of sperm function with nanotechnology tools.
Kameni SL, Dlamini NH, Feugang JM. Kameni SL, et al. Anim Reprod. 2024 Aug 16;21(3):e20240033. doi: 10.1590/1984-3143-AR2024-0033. eCollection 2024. Anim Reprod. 2024. PMID: 39176004 Free PMC article. - Advances in the preparation and biological applications of core@shell nanocrystals based on quantum dots and noble metal.
Wang X, Wang P, Li M, Li J. Wang X, et al. RSC Adv. 2024 Aug 20;14(36):26308-26324. doi: 10.1039/d4ra05386a. eCollection 2024 Aug 16. RSC Adv. 2024. PMID: 39165789 Free PMC article. Review. - Gold-Nanorod-Assisted Live Cell Nuclear Imaging Based on Near-Infrared II Dark-Field Microscopy.
Shi Y, Peng S, Huang Z, Feng Z, Liu W, Qian J, Zhou W. Shi Y, et al. Biology (Basel). 2023 Oct 31;12(11):1391. doi: 10.3390/biology12111391. Biology (Basel). 2023. PMID: 37997989 Free PMC article.
References
- Lim YT, et al. Selection of quantum dot wavelengths for biomedical assays and imaging. Mol Imaging. 2003;2:50–64. - PubMed
- Hatami F, et al. Carrier dynamics in type-II GaSb/GaAs quantum dots. Phys Rev B. 1998;57:4635–4641.
- Kim S, Fisher B, Eisler HJ, Bawendi M. Type-II quantum dots: CdTe/CdSe(core/shell) and CdSe/ZnTe(core/shell) heterostructures. J Am Chem Soc. 2003;125:11466–11467. - PubMed
- Jakub JW, Pendas S, Reintgen DS. Current status of sentinel lymph node mapping and biopsy: facts and controversies. Oncologist. 2003;8:59–68. - PubMed
- Bonnema J, Van DVCJ. Sentinel lymph node biopsy in breast cancer. Ann Oncol. 2002;13:1531–1537. - PubMed
Publication types
MeSH terms
Substances
Grants and funding
- R21 EB000673-01/EB/NIBIB NIH HHS/United States
- R33 EB000673/EB/NIBIB NIH HHS/United States
- R21 EB000673/EB/NIBIB NIH HHS/United States
- R33 EB000673-02/EB/NIBIB NIH HHS/United States
- R21 EB-00673/EB/NIBIB NIH HHS/United States
LinkOut - more resources
Full Text Sources
Other Literature Sources