Detection of enterococcal surface protein gene (esp) and amplified fragment length polymorphism typing of glycopeptide-resistant Enterococcus faecium during its emergence in a Greek intensive care unit - PubMed (original) (raw)

Detection of enterococcal surface protein gene (esp) and amplified fragment length polymorphism typing of glycopeptide-resistant Enterococcus faecium during its emergence in a Greek intensive care unit

C Routsi et al. J Clin Microbiol. 2003 Dec.

Abstract

The emergence of glycopeptide-resistant Enterococcus faecium (GREF) in a Greek intensive care unit was studied by amplified fragment length polymorphism analysis and esp gene detection. Three GREF clones harboring the esp gene were recovered from 17 out of 21 patients, indicating the dissemination of genetically homogenous and virulent strains of GREF.

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Figures

FIG. 1.

FIG. 1.

Dendrogram of the genetic similarity of all isolates by AFLP analysis. AFLP types are based on 90% genetic similarity. The presence (1) or absence (0) of the esp gene is indicated.

FIG. 2.

FIG. 2.

Admission of patients at the ICU. Bars indicate the preinfection period (open part) and infected period (solid part). The numbers on the left are the patient numbers.

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