The yeast Pdr15p ATP-binding cassette (ABC) protein is a general stress response factor implicated in cellular detoxification - PubMed (original) (raw)
. 2004 Mar 19;279(12):11593-9.
doi: 10.1074/jbc.M311282200. Epub 2003 Dec 29.
Affiliations
- PMID: 14699125
- DOI: 10.1074/jbc.M311282200
Free article
The yeast Pdr15p ATP-binding cassette (ABC) protein is a general stress response factor implicated in cellular detoxification
Hubert Wolfger et al. J Biol Chem. 2004.
Free article
Abstract
ATP-binding cassette (ABC) transporters play important roles in drug efflux, but some may also function in cellular detoxification. The Pdr15p ABC protein is the closest homologue of the multidrug efflux transporter Pdr5p, which mediates pleiotropic drug resistance to hundreds of unrelated compounds. In this study, we show that the plasma membrane protein Pdr15p displays limited drug transport capacity, mediating chloramphenicol and detergent tolerance. Interestingly, Pdr15p becomes most abundant when cells exit the exponential growth phase, whereas its closest homologue, Pdr5p, disappears after exponential growth. Furthermore, in contrast to Pdr5p, Pdr15p is strongly induced by various stress conditions including heat shock, low pH, weak acids, or high osmolarity. PDR15 induction bypasses the Pdr1p/Pdr3p regulators but requires the general stress regulator Msn2p, which directly decorates the stress response elements in the PDR15 promoter. Remarkably, however, Pdr15p induction bypasses upstream components of the high osmolarity glycerol (HOG) pathway including the Hog1p and Pbs2p kinases as well as the dedicated HOG cell surface sensors. Our data provide evidence for a novel upstream branch of the general stress response pathway activating Msn2p. In addition, the results demonstrate a cross-talk between stress response and the pleiotropic drug resistance network.
Similar articles
- Expression regulation of the yeast PDR5 ATP-binding cassette (ABC) transporter suggests a role in cellular detoxification during the exponential growth phase.
Mamnun YM, Schüller C, Kuchler K. Mamnun YM, et al. FEBS Lett. 2004 Feb 13;559(1-3):111-7. doi: 10.1016/S0014-5793(04)00046-8. FEBS Lett. 2004. PMID: 14960317 - The yeast zinc finger regulators Pdr1p and Pdr3p control pleiotropic drug resistance (PDR) as homo- and heterodimers in vivo.
Mamnun YM, Pandjaitan R, Mahé Y, Delahodde A, Kuchler K. Mamnun YM, et al. Mol Microbiol. 2002 Dec;46(5):1429-40. doi: 10.1046/j.1365-2958.2002.03262.x. Mol Microbiol. 2002. PMID: 12453227 - Negative transcriptional regulation of multidrug resistance gene expression by an Hsp70 protein.
Shahi P, Gulshan K, Moye-Rowley WS. Shahi P, et al. J Biol Chem. 2007 Sep 14;282(37):26822-26831. doi: 10.1074/jbc.M704772200. Epub 2007 Jul 18. J Biol Chem. 2007. PMID: 17636264 - Transcription factors and ABC transporters: from pleiotropic drug resistance to cellular signaling in yeast.
Buechel ER, Pinkett HW. Buechel ER, et al. FEBS Lett. 2020 Dec;594(23):3943-3964. doi: 10.1002/1873-3468.13964. Epub 2020 Nov 7. FEBS Lett. 2020. PMID: 33089887 Review. - Yeast ABC transporters-- a tale of sex, stress, drugs and aging.
Jungwirth H, Kuchler K. Jungwirth H, et al. FEBS Lett. 2006 Feb 13;580(4):1131-8. doi: 10.1016/j.febslet.2005.12.050. Epub 2005 Dec 22. FEBS Lett. 2006. PMID: 16406363 Review.
Cited by
- Engineering Saccharomyces cerevisiae for enhanced (-)-α-bisabolol production.
Jiang Y, Xia L, Gao S, Li N, Yu S, Zhou J. Jiang Y, et al. Synth Syst Biotechnol. 2023 Jan 20;8(2):187-195. doi: 10.1016/j.synbio.2023.01.004. eCollection 2023 Jun. Synth Syst Biotechnol. 2023. PMID: 36824492 Free PMC article. - Formate Dehydrogenase Improves the Resistance to Formic Acid and Acetic Acid Simultaneously in Saccharomyces cerevisiae.
Du C, Li Y, Xiang R, Yuan W. Du C, et al. Int J Mol Sci. 2022 Mar 21;23(6):3406. doi: 10.3390/ijms23063406. Int J Mol Sci. 2022. PMID: 35328826 Free PMC article. - Functional network motifs defined through integration of protein-protein and genetic interactions.
Sahoo A, Pechmann S. Sahoo A, et al. PeerJ. 2022 Feb 22;10:e13016. doi: 10.7717/peerj.13016. eCollection 2022. PeerJ. 2022. PMID: 35223214 Free PMC article. - Strain Degeneration in Pleurotus ostreatus: A Genotype Dependent Oxidative Stress Process Which Triggers Oxidative Stress, Cellular Detoxifying and Cell Wall Reshaping Genes.
Pérez G, Lopez-Moya F, Chuina E, Ibañez-Vea M, Garde E, López-Llorca LV, Pisabarro AG, Ramírez L. Pérez G, et al. J Fungi (Basel). 2021 Oct 14;7(10):862. doi: 10.3390/jof7100862. J Fungi (Basel). 2021. PMID: 34682283 Free PMC article. - Dissection of quantitative trait loci in the Lachancea waltii yeast species highlights major hotspots.
Peltier E, Bibi-Triki S, Dutreux F, Caradec C, Friedrich A, Llorente B, Schacherer J. Peltier E, et al. G3 (Bethesda). 2021 Sep 6;11(9):jkab242. doi: 10.1093/g3journal/jkab242. G3 (Bethesda). 2021. PMID: 34544138 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases