IgE enhances parasite clearance and regulates mast cell responses in mice infected with Trichinella spiralis - PubMed (original) (raw)

IgE enhances parasite clearance and regulates mast cell responses in mice infected with Trichinella spiralis

Michael F Gurish et al. J Immunol. 2004.

Abstract

Trichinella spiralis infection elicits a vigorous IgE response and pronounced intestinal and splenic mastocytosis in mice. Since IgE both activates mast cells (MC) and promotes their survival in culture, we examined its role in MC responses and parasite elimination in T. spiralis-infected mice. During primary infection, wild-type but not IgE-deficient (IgE(-/-)) BALB/c mice mounted a strong IgE response peaking 14 days into infection. The splenic mastocytosis observed in BALB/c mice following infection with T. spiralis was significantly diminished in IgE(-/-) mice while eosinophil responses were not diminished in either the blood or jejunum. Similar levels of peripheral blood eosinophilia and jejunal mastocytosis occurred in wild-type and IgE-deficient animals. Despite the normal MC response in the small intestine, serum levels of mouse MC protease-1 also were lower in parasite-infected IgE(-/-) animals and these animals were slower to eliminate the adult worms from the small intestine. The number of T. spiralis larvae present in the skeletal muscle of IgE(-/-) mice 28 days after primary infection was about twice that in BALB/c controls, and the fraction of larvae that was necrotic was reduced in the IgE-deficient animals. An intense deposition of IgE in and around the muscle larvae was observed in wild-type but not in IgE null mice. We conclude that IgE promotes parasite expulsion from the gut following T. spiralis infection and participates in the response to larval stages of the parasite. Furthermore, our observations support a role for IgE in the regulation of MC homeostasis in vivo.

PubMed Disclaimer

Cited by

Fasciolosis: pathogenesis, host-parasite interactions, and implication in vaccine development.
Flores-Velázquez LM, Ruiz-Campillo MT, Herrera-Torres G, Martínez-Moreno Á, Martínez-Moreno FJ, Zafra R, Buffoni L, Rufino-Moya PJ, Molina-Hernández V, Pérez J. Flores-Velázquez LM, et al. Front Vet Sci. 2023 Dec 11;10:1270064. doi: 10.3389/fvets.2023.1270064. eCollection 2023. Front Vet Sci. 2023. PMID: 38149297 Free PMC article. Review.
Total IgE as a serodiagnostic marker to aid murine fur mite detection.
Roble GS, Boteler W, Riedel E, Lipman NS. Roble GS, et al. J Am Assoc Lab Anim Sci. 2012 Mar;51(2):199-208. J Am Assoc Lab Anim Sci. 2012. PMID: 22776120 Free PMC article.
Mast cell-orchestrated immunity to pathogens.
Abraham SN, St John AL. Abraham SN, et al. Nat Rev Immunol. 2010 Jun;10(6):440-52. doi: 10.1038/nri2782. Nat Rev Immunol. 2010. PMID: 20498670 Free PMC article. Review.
IgE Antibodies: From Structure to Function and Clinical Translation.
Sutton BJ, Davies AM, Bax HJ, Karagiannis SN. Sutton BJ, et al. Antibodies (Basel). 2019 Feb 22;8(1):19. doi: 10.3390/antib8010019. Antibodies (Basel). 2019. PMID: 31544825 Free PMC article. Review.
Regulatory roles of mast cells in immune responses.
Morita H, Saito H, Matsumoto K, Nakae S. Morita H, et al. Semin Immunopathol. 2016 Sep;38(5):623-9. doi: 10.1007/s00281-016-0566-0. Epub 2016 May 6. Semin Immunopathol. 2016. PMID: 27154294 Review.

IgE enhances parasite clearance and regulates mast cell responses in mice infected with Trichinella spiralis - PubMed (original) (raw)