Distinct roles of inositol 1,4,5-trisphosphate receptor types 1 and 3 in Ca2+ signaling - PubMed (original) (raw)
. 2004 Mar 19;279(12):11967-75.
doi: 10.1074/jbc.M311456200. Epub 2004 Jan 5.
Affiliations
- PMID: 14707143
- DOI: 10.1074/jbc.M311456200
Free article
Distinct roles of inositol 1,4,5-trisphosphate receptor types 1 and 3 in Ca2+ signaling
Mitsuharu Hattori et al. J Biol Chem. 2004.
Free article
Abstract
Three subtypes of inositol 1,4,5-trisphosphate receptor (IP(3)R1, IP(3)R2, and IP(3)R3) Ca(2+) release channel share basic properties but differ in terms of regulation. To what extent they contribute to complex Ca(2+) signaling, such as Ca(2+) oscillations, remains largely unknown. Here we show that HeLa cells express comparable amounts of IP(3)R1 and IP(3)R3, but knockdown by RNA interference of each subtype results in dramatically distinct Ca(2+) signaling patterns. Knockdown of IP(3)R1 significantly decreases total Ca(2+) signals and terminates Ca(2+) oscillations. Conversely, knockdown of IP(3)R3 leads to more robust and long lasting Ca(2+) oscillations than in controls. Effects of IP(3)R3 knockdown are surprisingly similar in COS-7 cells that predominantly (>90% of total IP(3)R) express IP(3)R3, suggesting that IP(3)R3 functions as an anti-Ca(2+)-oscillatory unit without contributing to peak amplitude of Ca(2+) signals, irrespective of its relative expression level. Therefore, differential expression of the IP(3)R subtype is critical for various forms of Ca(2+) signaling, and, particularly, IP(3)R1 and IP(3)R3 have opposite roles in generating Ca(2+) oscillations.
Similar articles
- Differential modulation of inositol 1,4,5-trisphosphate receptor type 1 and type 3 by ATP.
Maes K, Missiaen L, De Smet P, Vanlingen S, Callewaert G, Parys JB, De Smedt H. Maes K, et al. Cell Calcium. 2000 May;27(5):257-67. doi: 10.1054/ceca.2000.0121. Cell Calcium. 2000. PMID: 10859592 - Functional analysis of the green fluorescent protein-tagged inositol 1,4,5-trisphosphate receptor type 3 in Ca(2+) release and entry in DT40 B lymphocytes.
Morita T, Tanimura A, Nezu A, Kurosaki T, Tojyo Y. Morita T, et al. Biochem J. 2004 Sep 15;382(Pt 3):793-801. doi: 10.1042/BJ20031970. Biochem J. 2004. PMID: 15175012 Free PMC article. - Different roles of inositol 1,4,5-trisphosphate receptor subtypes in prostaglandin F(2alpha)-induced calcium oscillations and pacemaking activity of NRK fibroblasts.
Almirza WH, Peters PH, van Meerwijk WP, van Zoelen EJ, Theuvenet AP. Almirza WH, et al. Cell Calcium. 2010 Jun;47(6):544-53. doi: 10.1016/j.ceca.2010.05.004. Epub 2010 Jun 9. Cell Calcium. 2010. PMID: 20542562 - Structural insights into the regulatory mechanism of IP3 receptor.
Bosanac I, Michikawa T, Mikoshiba K, Ikura M. Bosanac I, et al. Biochim Biophys Acta. 2004 Dec 6;1742(1-3):89-102. doi: 10.1016/j.bbamcr.2004.09.016. Biochim Biophys Acta. 2004. PMID: 15590059 Review. - IP3 receptors and their regulation by calmodulin and cytosolic Ca2+.
Taylor CW, Laude AJ. Taylor CW, et al. Cell Calcium. 2002 Nov-Dec;32(5-6):321-34. doi: 10.1016/s0143416002001859. Cell Calcium. 2002. PMID: 12543092 Review.
Cited by
- Steady-state regulation of COPII-dependent secretory cargo sorting by inositol trisphosphate receptors, calcium, and penta EF hand proteins.
Held A, Lapka J, Sargeant J, Hojanazarova J, Shaheen A, Galindo S, Madreiter-Sokolowski C, Malli R, Graier WF, Hay JC. Held A, et al. J Biol Chem. 2023 Dec;299(12):105471. doi: 10.1016/j.jbc.2023.105471. Epub 2023 Nov 17. J Biol Chem. 2023. PMID: 37979918 Free PMC article. - Inhibition of adenylyl cyclase 1 by ST034307 inhibits IP3-evoked changes in sino-atrial node beat rate.
Bose SJ, Read MJ, Akerman E, Capel RA, Ayagama T, Russell A, Terrar DA, Zaccolo M, Burton RAB. Bose SJ, et al. Front Pharmacol. 2022 Aug 29;13:951897. doi: 10.3389/fphar.2022.951897. eCollection 2022. Front Pharmacol. 2022. PMID: 36105228 Free PMC article. - KRAP is required for diffuse and punctate IP3-mediated Ca2+ liberation and determines the number of functional IP3R channels within clusters.
Vorontsova I, Lock JT, Parker I. Vorontsova I, et al. Cell Calcium. 2022 Nov;107:102638. doi: 10.1016/j.ceca.2022.102638. Epub 2022 Aug 19. Cell Calcium. 2022. PMID: 36030740 Free PMC article. - IRAG2 Interacts with IP3-Receptor Types 1, 2, and 3 and Regulates Intracellular Ca2+ in Murine Pancreatic Acinar Cells.
Prüschenk S, Majer M, Schreiber R, Schlossmann J. Prüschenk S, et al. Int J Mol Sci. 2021 Dec 14;22(24):13409. doi: 10.3390/ijms222413409. Int J Mol Sci. 2021. PMID: 34948204 Free PMC article. - Spatiotemporal regulation of store-operated calcium entry in cancer metastasis.
Lu F, Li Y, Lin S, Cheng H, Yang S. Lu F, et al. Biochem Soc Trans. 2021 Dec 17;49(6):2581-2589. doi: 10.1042/BST20210307. Biochem Soc Trans. 2021. PMID: 34854917 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous