Imaging skeletal pathology in mutant mice by microcomputed tomography - PubMed (original) (raw)
. 2003 Dec;30(12):2659-65.
Affiliations
- PMID: 14719210
Imaging skeletal pathology in mutant mice by microcomputed tomography
Alice F Ford-Hutchinson et al. J Rheumatol. 2003 Dec.
Abstract
Objective: We describe the utility of microcomputed tomography ( micro CT) for imaging skeletal abnormalities in rodent model systems. For the purpose of illustration, the progressive ankylosis (ank) mutant was selected. ank mice develop prominent articular and periarticular calcifications at multiple anatomical sites, including paws, elbows, knees, and vertebrae.
Methods: Forelimbs, hindlimbs, and proximal tail vertebrae of 4-month-old female ank/ank mice were scanned at 15 micro m resolution using a SkyScan 1072 micro CT instrument and images were generated using Analyze 4.0 software.
Results: This technique was able to show, in 3-dimensional images, the abnormal calcification of ank/ank mice, which was readily observed within joint surfaces, on periosteal surfaces, sesamoid bones, menisci, and joint capsules, as well as other periarticular ligamentous structures.
Conclusion: As illustrated by the example of the progressive ankylosis mutant, micro CT represents a powerful emerging tool for identifying and monitoring the progression of developmental or acquired skeletal abnormalities within rodent models.
Similar articles
- Microcomputed tomography imaging of skeletal development and growth.
Guldberg RE, Lin AS, Coleman R, Robertson G, Duvall C. Guldberg RE, et al. Birth Defects Res C Embryo Today. 2004 Sep;72(3):250-9. doi: 10.1002/bdrc.20016. Birth Defects Res C Embryo Today. 2004. PMID: 15495187 Review. - Progressive ankylosis, a new skeletal mutation in the mouse.
Sweet HO, Green MC. Sweet HO, et al. J Hered. 1981 Mar-Apr;72(2):87-93. doi: 10.1093/oxfordjournals.jhered.a109459. J Hered. 1981. PMID: 7276519 - HLA-B27 has no effect on the phenotypic expression of progressive ankylosis in ank/ank mice.
Krug HE, Taurog JD. Krug HE, et al. J Rheumatol. 2000 May;27(5):1257-9. J Rheumatol. 2000. PMID: 10813297 - Ultrastructure of the mineralizing metacarpophalangeal joint of progressive ankylosis (ank/ank) mice.
Sampson HW. Sampson HW. Am J Anat. 1988 Jul;182(3):257-69. doi: 10.1002/aja.1001820307. Am J Anat. 1988. PMID: 3213824 - [Analysis of trabecular microstructure using micro-computed tomography].
Ito M. Ito M. Nihon Rinsho. 1998 Jun;56(6):1484-90. Nihon Rinsho. 1998. PMID: 9648470 Review. Japanese.
Cited by
- Rapid bone staining with hair removal (RAP-B/HR): a non-destructive and rapid whole-mount bone staining protocol optimized for adult hairy mice.
Kariyama N, Sakata-Haga H, Tsukada T, Shimada H, Taniguchi M, Hatta T. Kariyama N, et al. Sci Rep. 2021 Jan 21;11(1):1950. doi: 10.1038/s41598-021-81616-7. Sci Rep. 2021. PMID: 33479385 Free PMC article. - Quantitative in vivo micro-computed tomography for assessment of age-dependent changes in murine whole-body composition.
Beaucage KL, Pollmann SI, Sims SM, Dixon SJ, Holdsworth DW. Beaucage KL, et al. Bone Rep. 2016 Apr 9;5:70-80. doi: 10.1016/j.bonr.2016.04.002. eCollection 2016 Dec. Bone Rep. 2016. PMID: 28326349 Free PMC article. - N-ethyl-N-Nitrosourea (ENU) induced mutations within the klotho gene lead to ectopic calcification and reduced lifespan in mouse models.
Esapa CT, Hannan FM, Babinsky VN, Potter P, Thomas GP, Croucher PI, Brown MA, Brown SD, Cox RD, Thakker RV. Esapa CT, et al. PLoS One. 2015 Apr 10;10(4):e0122650. doi: 10.1371/journal.pone.0122650. eCollection 2015. PLoS One. 2015. PMID: 25860694 Free PMC article. - Integration of comprehensive 3D microCT and signaling analysis reveals differential regulatory mechanisms of craniofacial bone development.
Ho TV, Iwata J, Ho HA, Grimes WC, Park S, Sanchez-Lara PA, Chai Y. Ho TV, et al. Dev Biol. 2015 Apr 15;400(2):180-90. doi: 10.1016/j.ydbio.2015.02.010. Epub 2015 Feb 23. Dev Biol. 2015. PMID: 25722190 Free PMC article. - Quantification of bone changes in a collagen-induced arthritis mouse model by reconstructed three dimensional micro-CT.
Yang S, Hollister AM, Orchard EA, Chaudhery SI, Ostanin DV, Lokitz SJ, Mathis JM. Yang S, et al. Biol Proced Online. 2013 Jul 15;15:8. doi: 10.1186/1480-9222-15-8. eCollection 2013. Biol Proced Online. 2013. PMID: 23855709 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Medical
Research Materials