Coated-vesicle formation in vitro: conflicting results using different assays - PubMed (original) (raw)
Coated-vesicle formation in vitro: conflicting results using different assays
S L Schmid. Trends Cell Biol. 1993 May.
Abstract
Cell-free systems provide essential tools for elucidating the molecular mechanisms underlying complex cellular processes such as vesicular transport. The biochemical utility of these model systems is strengthened by assays that allow rapid, quantitative detection of the events being studied. Two model systems have recently been developed to reconstitute coated-vesicle budding, and two different biochemical assays are used to detect this event. Striking differences in the biochemical requirements for 'coated-vesicle budding' are detected by these two assays, suggesting that two distinct events are being measured. These findings have wide implications for the use of cell-free assay systems in cell biology.
Similar articles
- In vitro reconstitution of discrete stages of dynamin-dependent endocytosis.
Higgins MK, McMahon HT. Higgins MK, et al. Methods Enzymol. 2005;404:597-611. doi: 10.1016/S0076-6879(05)04052-8. Methods Enzymol. 2005. PMID: 16413303 - Cell-free transport to distinct Golgi cisternae is compartment specific and ARF independent.
Happe S, Weidman P. Happe S, et al. J Cell Biol. 1998 Feb 9;140(3):511-23. doi: 10.1083/jcb.140.3.511. J Cell Biol. 1998. PMID: 9456313 Free PMC article. - Clathrin-coated vesicle formation and protein sorting: an integrated process.
Schmid SL. Schmid SL. Annu Rev Biochem. 1997;66:511-48. doi: 10.1146/annurev.biochem.66.1.511. Annu Rev Biochem. 1997. PMID: 9242916 Review. - A rapid and quantitative coat protein complex II vesicle formation assay using luciferase reporters.
Fromme JC, Kim J. Fromme JC, et al. Anal Biochem. 2012 Feb 15;421(2):482-8. doi: 10.1016/j.ab.2011.12.030. Epub 2011 Dec 24. Anal Biochem. 2012. PMID: 22244805 - Biochemical requirements for the formation of clathrin- and COP-coated transport vesicles.
Schmid SL. Schmid SL. Curr Opin Cell Biol. 1993 Aug;5(4):621-7. doi: 10.1016/0955-0674(93)90131-9. Curr Opin Cell Biol. 1993. PMID: 8257603 Review.
Cited by
- The kinetic aspects of intracellular fluorescence labeling with TMA-DPH support the maturation model for endocytosis in L929 cells.
Illinger D, Kuhry JG. Illinger D, et al. J Cell Biol. 1994 May;125(4):783-94. doi: 10.1083/jcb.125.4.783. J Cell Biol. 1994. PMID: 8188746 Free PMC article. - Induction of mutant dynamin specifically blocks endocytic coated vesicle formation.
Damke H, Baba T, Warnock DE, Schmid SL. Damke H, et al. J Cell Biol. 1994 Nov;127(4):915-34. doi: 10.1083/jcb.127.4.915. J Cell Biol. 1994. PMID: 7962076 Free PMC article. - Acute cholesterol depletion inhibits clathrin-coated pit budding.
Subtil A, Gaidarov I, Kobylarz K, Lampson MA, Keen JH, McGraw TE. Subtil A, et al. Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6775-80. doi: 10.1073/pnas.96.12.6775. Proc Natl Acad Sci U S A. 1999. PMID: 10359788 Free PMC article. - Mutations in human dynamin block an intermediate stage in coated vesicle formation.
van der Bliek AM, Redelmeier TE, Damke H, Tisdale EJ, Meyerowitz EM, Schmid SL. van der Bliek AM, et al. J Cell Biol. 1993 Aug;122(3):553-63. doi: 10.1083/jcb.122.3.553. J Cell Biol. 1993. PMID: 8101525 Free PMC article. - Presynaptic mitochondria and the temporal pattern of neurotransmitter release.
Brodin L, Bakeeva L, Shupliakov O. Brodin L, et al. Philos Trans R Soc Lond B Biol Sci. 1999 Feb 28;354(1381):365-72. doi: 10.1098/rstb.1999.0388. Philos Trans R Soc Lond B Biol Sci. 1999. PMID: 10212485 Free PMC article.