Laser-assisted microdissection: methods for the molecular analysis of psychiatric disorders at a cellular resolution - PubMed (original) (raw)
Comparative Study
Laser-assisted microdissection: methods for the molecular analysis of psychiatric disorders at a cellular resolution
Philip W J Burnet et al. Biol Psychiatry. 2004.
Abstract
Gene expression arrays and proteomics together provide a great opportunity to reveal the molecular pathophysiology of psychiatric disorders; however, their potential will not be realized unless due attention is paid to the cellular heterogeneity of the brain and the likely differential neuropathological involvement of specific neuronal and glial cell types. Hence, methods are needed which can procure homogeneous populations of cells as a source of messenger RNA, protein, or DNA. Laser-assisted microdissection techniques provide such a tool. Here we briefly discuss the principles, applications, and limitations of laser-assisted microdissection in psychiatric research.
Similar articles
- Laser-assisted microdissection: applications in molecular pathology.
Sirivatanauksorn Y, Drury R, Crnogorac-Jurcević T, Sirivatanauksorn V, Lemoine NR. Sirivatanauksorn Y, et al. J Pathol. 1999 Oct;189(2):150-4. doi: 10.1002/(SICI)1096-9896(199910)189:2<150::AID-PATH451>3.0.CO;2-G. J Pathol. 1999. PMID: 10547567 Review. - Laser capture microdissection in pathology.
Fend F, Raffeld M. Fend F, et al. J Clin Pathol. 2000 Sep;53(9):666-72. doi: 10.1136/jcp.53.9.666. J Clin Pathol. 2000. PMID: 11041055 Free PMC article. Review. - Laser capture microdissection: methodical aspects and applications with emphasis on immuno-laser capture microdissection.
Fend F, Kremer M, Quintanilla-Martinez L. Fend F, et al. Pathobiology. 2000;68(4-5):209-14. doi: 10.1159/000055925. Pathobiology. 2000. PMID: 11279348 Review. - [New developments in application of laser capture microdissection].
Yan F, Li ZF. Yan F, et al. Ai Zheng. 2004 Jul;23(7):860-4. Ai Zheng. 2004. PMID: 15248929 Review. Chinese. - Laser capture microdissection as an aid to ultrastructural analysis.
Grant K, Jerome WG. Grant K, et al. Microsc Microanal. 2002 Jun;8(3):170-5. doi: 10.1017/s143192760202010x. Microsc Microanal. 2002. PMID: 12539787
Cited by
- Enrichment of single neurons and defined brain regions from human brain tissue samples for subsequent proteome analysis.
Molina M, Steinbach S, Park YM, Yun SY, Di Lorenzo Alho AT, Heinsen H, Grinberg LT, Marcus K, Leite RE, May C. Molina M, et al. J Neural Transm (Vienna). 2015 Jul;122(7):993-1005. doi: 10.1007/s00702-015-1414-4. Epub 2015 Jun 30. J Neural Transm (Vienna). 2015. PMID: 26123835 - Application of NeuroTrace staining in the fresh frozen brain samples to laser microdissection combined with quantitative RT-PCR analysis.
Benner S, Kakeyama M, Endo T, Yoshioka W, Tohyama C. Benner S, et al. BMC Res Notes. 2015 Jun 20;8:252. doi: 10.1186/s13104-015-1222-9. BMC Res Notes. 2015. PMID: 26092293 Free PMC article. - Laser capture microdissection: Big data from small samples.
Datta S, Malhotra L, Dickerson R, Chaffee S, Sen CK, Roy S. Datta S, et al. Histol Histopathol. 2015 Nov;30(11):1255-69. doi: 10.14670/HH-11-622. Epub 2015 Apr 20. Histol Histopathol. 2015. PMID: 25892148 Free PMC article. Review. - GABA Targets for the Treatment of Cognitive Dysfunction in Schizophrenia.
Volk DW, Lewis DA. Volk DW, et al. Curr Neuropharmacol. 2005 Jan;3(1):45-62. doi: 10.2174/1570159052773396. Curr Neuropharmacol. 2005. PMID: 22545031 Free PMC article. - Laser capture microdissection in the tissue biorepository.
Liu A. Liu A. J Biomol Tech. 2010 Sep;21(3):120-5. J Biomol Tech. 2010. PMID: 20808641 Free PMC article. Review.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Medical