Kinetic determinants of high-fidelity tRNA discrimination on the ribosome - PubMed (original) (raw)
Kinetic determinants of high-fidelity tRNA discrimination on the ribosome
Kirill B Gromadski et al. Mol Cell. 2004.
Free article
Abstract
The ribosome selects aminoacyl-tRNA (aa-tRNA) matching to the mRNA codon from the bulk of non-matching aa-tRNAs in two consecutive selection steps, initial selection and proofreading. Here we report the kinetic analysis of selection taking place under conditions where the overall selectivity was close to values observed in vivo and initial selection and proofreading contributed about equally. Comparison of the rate constants shows that the 350-fold difference in stabilities of cognate and near-cognate codon-anticodon complexes is not used for tRNA selection due to high rate of GTP hydrolysis in the cognate complex. tRNA selection at the initial selection step is entirely kinetically controlled and is due to much faster (650-fold) GTP hydrolysis of cognate compared to near-cognate substrate.
Similar articles
- Induced fit in initial selection and proofreading of aminoacyl-tRNA on the ribosome.
Pape T, Wintermeyer W, Rodnina M. Pape T, et al. EMBO J. 1999 Jul 1;18(13):3800-7. doi: 10.1093/emboj/18.13.3800. EMBO J. 1999. PMID: 10393195 Free PMC article. - Cryo-EM of elongating ribosome with EF-Tu•GTP elucidates tRNA proofreading.
Loveland AB, Demo G, Korostelev AA. Loveland AB, et al. Nature. 2020 Aug;584(7822):640-645. doi: 10.1038/s41586-020-2447-x. Epub 2020 Jul 1. Nature. 2020. PMID: 32612237 Free PMC article. - Fidelity of aminoacyl-tRNA selection on the ribosome: kinetic and structural mechanisms.
Rodnina MV, Wintermeyer W. Rodnina MV, et al. Annu Rev Biochem. 2001;70:415-35. doi: 10.1146/annurev.biochem.70.1.415. Annu Rev Biochem. 2001. PMID: 11395413 Review. - Evolutionary optimization of speed and accuracy of decoding on the ribosome.
Wohlgemuth I, Pohl C, Mittelstaet J, Konevega AL, Rodnina MV. Wohlgemuth I, et al. Philos Trans R Soc Lond B Biol Sci. 2011 Oct 27;366(1580):2979-86. doi: 10.1098/rstb.2011.0138. Philos Trans R Soc Lond B Biol Sci. 2011. PMID: 21930591 Free PMC article. Review.
Cited by
- Translational recoding: canonical translation mechanisms reinterpreted.
Rodnina MV, Korniy N, Klimova M, Karki P, Peng BZ, Senyushkina T, Belardinelli R, Maracci C, Wohlgemuth I, Samatova E, Peske F. Rodnina MV, et al. Nucleic Acids Res. 2020 Feb 20;48(3):1056-1067. doi: 10.1093/nar/gkz783. Nucleic Acids Res. 2020. PMID: 31511883 Free PMC article. Review. - The energy cost and optimal design of networks for biological discrimination.
Yu Q, Kolomeisky AB, Igoshin OA. Yu Q, et al. J R Soc Interface. 2022 Mar;19(188):20210883. doi: 10.1098/rsif.2021.0883. Epub 2022 Mar 9. J R Soc Interface. 2022. PMID: 35259959 Free PMC article. - The mechanism of error induction by the antibiotic viomycin provides insight into the fidelity mechanism of translation.
Holm M, Mandava CS, Ehrenberg M, Sanyal S. Holm M, et al. Elife. 2019 Jun 7;8:e46124. doi: 10.7554/eLife.46124. Elife. 2019. PMID: 31172942 Free PMC article. - Cellular mechanisms that control mistranslation.
Reynolds NM, Lazazzera BA, Ibba M. Reynolds NM, et al. Nat Rev Microbiol. 2010 Dec;8(12):849-56. doi: 10.1038/nrmicro2472. Nat Rev Microbiol. 2010. PMID: 21079633 Review. - Explaining complex codon usage patterns with selection for translational efficiency, mutation bias, and genetic drift.
Shah P, Gilchrist MA. Shah P, et al. Proc Natl Acad Sci U S A. 2011 Jun 21;108(25):10231-6. doi: 10.1073/pnas.1016719108. Epub 2011 Jun 6. Proc Natl Acad Sci U S A. 2011. PMID: 21646514 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources