A poly(A) tail-responsive in vitro system for cap- or IRES-driven translation from HeLa cells - PubMed (original) (raw)

A poly(A) tail-responsive in vitro system for cap- or IRES-driven translation from HeLa cells

Christian Thoma et al. Methods Mol Biol. 2004.

Abstract

In cells, the poly(A) tail stimulates translation from messenger RNAs bearing a cap structure or viral IRES elements. This 3' end-mediated stimulation of translation is not reflected in commonly used commercial cell-free translation systems prepared from rabbit reticulocytes or wheat germ. We describe a simple procedure to generate poly(A) tail-responsive translation extracts from HeLa cells. We suggest that this procedure should be adaptable to many animal cell lines.

PubMed Disclaimer

Similar articles

• Picornavirus IRESes and the poly(A) tail jointly promote cap-independent translation in a mammalian cell-free system.
  Bergamini G, Preiss T, Hentze MW. Bergamini G, et al. RNA. 2000 Dec;6(12):1781-90. doi: 10.1017/s1355838200001679. RNA. 2000. PMID: 11142378 Free PMC article.
• Poly(A)-binding protein interaction with elF4G stimulates picornavirus IRES-dependent translation.
  Svitkin YV, Imataka H, Khaleghpour K, Kahvejian A, Liebig HD, Sonenberg N. Svitkin YV, et al. RNA. 2001 Dec;7(12):1743-52. RNA. 2001. PMID: 11780631 Free PMC article.
• Cap-Poly(A) synergy in mammalian cell-free extracts. Investigation of the requirements for poly(A)-mediated stimulation of translation initiation.
  Michel YM, Poncet D, Piron M, Kean KM, Borman AM. Michel YM, et al. J Biol Chem. 2000 Oct 13;275(41):32268-76. doi: 10.1074/jbc.M004304200. J Biol Chem. 2000. PMID: 10922367
• Structural and mechanistic insights into hepatitis C viral translation initiation.
  Fraser CS, Doudna JA. Fraser CS, et al. Nat Rev Microbiol. 2007 Jan;5(1):29-38. doi: 10.1038/nrmicro1558. Epub 2006 Nov 27. Nat Rev Microbiol. 2007. PMID: 17128284 Review.
• An atypical IRES within the 5' UTR of a dicistrovirus genome.
  Roberts LO, Groppelli E. Roberts LO, et al. Virus Res. 2009 Feb;139(2):157-65. doi: 10.1016/j.virusres.2008.07.017. Epub 2008 Sep 11. Virus Res. 2009. PMID: 18755228 Review.

Cited by

• A highly optimized human in vitro translation system.
  Bothe A, Ban N. Bothe A, et al. Cell Rep Methods. 2024 Apr 22;4(4):100755. doi: 10.1016/j.crmeth.2024.100755. Epub 2024 Apr 11. Cell Rep Methods. 2024. PMID: 38608690 Free PMC article.
• Human-rabbit Hybrid Translation System to Explore the Function of Modified Ribosomes.
  Matsuura-Suzuki E, Toh H, Iwasaki S. Matsuura-Suzuki E, et al. Bio Protoc. 2023 Jul 5;13(13):e4714. doi: 10.21769/BioProtoc.4714. eCollection 2023 Jul 5. Bio Protoc. 2023. PMID: 37456340 Free PMC article.
• Crystal Structure of a Variant PAM2 Motif of LARP4B Bound to the MLLE Domain of PABPC1.
  Grimm C, Pelz JP, Schneider C, Schäffler K, Fischer U. Grimm C, et al. Biomolecules. 2020 Jun 6;10(6):872. doi: 10.3390/biom10060872. Biomolecules. 2020. PMID: 32517187 Free PMC article.
• A researcher's guide to the galaxy of IRESs.
  Terenin IM, Smirnova VV, Andreev DE, Dmitriev SE, Shatsky IN. Terenin IM, et al. Cell Mol Life Sci. 2017 Apr;74(8):1431-1455. doi: 10.1007/s00018-016-2409-5. Epub 2016 Nov 16. Cell Mol Life Sci. 2017. PMID: 27853833 Free PMC article. Review.
• Cap homeostasis is independent of poly(A) tail length.
  Kiss DL, Oman KM, Dougherty JA, Mukherjee C, Bundschuh R, Schoenberg DR. Kiss DL, et al. Nucleic Acids Res. 2016 Jan 8;44(1):304-14. doi: 10.1093/nar/gkv1460. Epub 2015 Dec 15. Nucleic Acids Res. 2016. PMID: 26673707 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Springer

• Other Literature Sources

  • The Lens - Patent Citations

• Miscellaneous

  • NCI CPTAC Assay Portal