SOCS3 is a critical physiological negative regulator of G-CSF signaling and emergency granulopoiesis - PubMed (original) (raw)

doi: 10.1016/s1074-7613(04)00022-6.

Donald Metcalf, Lorraine Robb, Wei Wei, Sandra Mifsud, Ladina DiRago, Leonie A Cluse, Kate D Sutherland, Lynne Hartley, Emily Williams, Jian-Guo Zhang, Douglas J Hilton, Nicos A Nicola, Warren S Alexander, Andrew W Roberts

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SOCS3 is a critical physiological negative regulator of G-CSF signaling and emergency granulopoiesis

Ben A Croker et al. Immunity. 2004 Feb.

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Abstract

To determine the importance of suppressor of cytokine signaling-3 (SOCS3) in the regulation of hematopoietic growth factor signaling generally, and of G-CSF-induced cellular responses specifically, we created mice in which the Socs3 gene was deleted in all hematopoietic cells. Although normal until young adulthood, these mice then developed neutrophilia and a spectrum of inflammatory pathologies. When stimulated with G-CSF in vitro, SOCS3-deficient cells of the neutrophilic granulocyte lineage exhibited prolonged STAT3 activation and enhanced cellular responses to G-CSF, including an increase in cloning frequency, survival, and proliferative capacity. Consistent with the in vitro findings, mutant mice injected with G-CSF displayed enhanced neutrophilia, progenitor cell mobilization, and splenomegaly, but unexpectedly also developed inflammatory neutrophil infiltration into multiple tissues and consequent hind-leg paresis. We conclude that SOCS3 is a key negative regulator of G-CSF signaling in myeloid cells and that this is of particular significance during G-CSF-driven emergency granulopoiesis.

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