Accumulation of NH2-terminal fragment of connective tissue growth factor in the vitreous of patients with proliferative diabetic retinopathy - PubMed (original) (raw)
Accumulation of NH2-terminal fragment of connective tissue growth factor in the vitreous of patients with proliferative diabetic retinopathy
David R Hinton et al. Diabetes Care. 2004 Mar.
Abstract
Objective: To evaluate the expression of connective tissue growth factor (CTGF) and its fragments in the vitreous of patients with proliferative diabetic retinopathy (PDR) and to localize CTGF expression in associated preretinal membranes.
Research design and methods: Vitreous was obtained from 24 patients with active PDR, 4 patients with quiescent PDR, and 23 patients with other retinal diseases and no diabetes, including 5 patients with vitreous hemorrhage. Enzyme-linked immunosorbent assay was used to determine levels of whole CTGF and its NH2- and COOH-terminal fragments. Preretinal membranes from three patients with active PDR were stained immunohistochemically for the presence of CTGF and cell type-specific markers.
Results: A significant increase in NH2-terminal CTGF fragment content was found in vitreous samples from patients with active PDR when compared with samples from nondiabetic patients (P<0.0001) or patients with quiescent PDR (P=0.02). Levels of NH2-terminal CTGF were also greater in vitreous samples from diabetic patients with vitreous hemorrhage compared with samples from nondiabetic patients with vitreous hemorrhage (P=0.02). Vitreous levels of whole CTGF were similar in all groups. COOH-terminal fragments of CTGF were not detected. CTGF immunoreactivity was predominantly localized to smooth muscle actin-positive myofibroblasts within active PDR membranes.
Conclusions: -NH2-terminal CTGF fragment content is increased in the vitreous of patients with active PDR, suggesting that it plays a pathogenic role or represents a surrogate marker of CTGF activity in the disorder. The localization of CTGF in myofibroblasts suggests a local paracrine mechanism for induction of fibrosis and neovascularization.
Similar articles
- The angio-fibrotic switch of VEGF and CTGF in proliferative diabetic retinopathy.
Kuiper EJ, Van Nieuwenhoven FA, de Smet MD, van Meurs JC, Tanck MW, Oliver N, Klaassen I, Van Noorden CJ, Goldschmeding R, Schlingemann RO. Kuiper EJ, et al. PLoS One. 2008 Jul 16;3(7):e2675. doi: 10.1371/journal.pone.0002675. PLoS One. 2008. PMID: 18628999 Free PMC article. - Expression of angiogenic and fibrogenic factors in proliferative vitreoretinal disorders.
Abu El-Asrar AM, Van den Steen PE, Al-Amro SA, Missotten L, Opdenakker G, Geboes K. Abu El-Asrar AM, et al. Int Ophthalmol. 2007 Feb;27(1):11-22. doi: 10.1007/s10792-007-9053-x. Epub 2007 Mar 21. Int Ophthalmol. 2007. PMID: 17375263 - Vitreous levels of placental growth factor correlate with activity of proliferative diabetic retinopathy and are not influenced by bevacizumab treatment.
Al Kahtani E, Xu Z, Al Rashaed S, Wu L, Mahale A, Tian J, Abboud EB, Ghazi NG, Kozak I, Gupta V, Arevalo JF, Duh EJ. Al Kahtani E, et al. Eye (Lond). 2017 Apr;31(4):529-536. doi: 10.1038/eye.2016.246. Epub 2016 Nov 25. Eye (Lond). 2017. PMID: 27886182 Free PMC article. - Human vitreous in proliferative diabetic retinopathy: Characterization and translational implications.
Nawaz IM, Rezzola S, Cancarini A, Russo A, Costagliola C, Semeraro F, Presta M. Nawaz IM, et al. Prog Retin Eye Res. 2019 Sep;72:100756. doi: 10.1016/j.preteyeres.2019.03.002. Epub 2019 Apr 2. Prog Retin Eye Res. 2019. PMID: 30951889 Review. - Research progress on the role of connective tissue growth factor in fibrosis of diabetic retinopathy.
Ma T, Dong LJ, Du XL, Niu R, Hu BJ. Ma T, et al. Int J Ophthalmol. 2018 Sep 18;11(9):1550-1554. doi: 10.18240/ijo.2018.09.20. eCollection 2018. Int J Ophthalmol. 2018. PMID: 30225233 Free PMC article. Review.
Cited by
- Connective tissue growth factor regulates retinal neovascularization through p53 protein-dependent transactivation of the matrix metalloproteinase (MMP)-2 gene.
Chintala H, Liu H, Parmar R, Kamalska M, Kim YJ, Lovett D, Grant MB, Chaqour B. Chintala H, et al. J Biol Chem. 2012 Nov 23;287(48):40570-85. doi: 10.1074/jbc.M112.386565. Epub 2012 Oct 9. J Biol Chem. 2012. PMID: 23048035 Free PMC article. - Angiogenesis is not impaired in connective tissue growth factor (CTGF) knock-out mice.
Kuiper EJ, Roestenberg P, Ehlken C, Lambert V, van Treslong-de Groot HB, Lyons KM, Agostini HJ, Rakic JM, Klaassen I, Van Noorden CJ, Goldschmeding R, Schlingemann RO. Kuiper EJ, et al. J Histochem Cytochem. 2007 Nov;55(11):1139-47. doi: 10.1369/jhc.7A7258.2007. Epub 2007 Jul 11. J Histochem Cytochem. 2007. PMID: 17625227 Free PMC article. - The angio-fibrotic switch of VEGF and CTGF in proliferative diabetic retinopathy.
Kuiper EJ, Van Nieuwenhoven FA, de Smet MD, van Meurs JC, Tanck MW, Oliver N, Klaassen I, Van Noorden CJ, Goldschmeding R, Schlingemann RO. Kuiper EJ, et al. PLoS One. 2008 Jul 16;3(7):e2675. doi: 10.1371/journal.pone.0002675. PLoS One. 2008. PMID: 18628999 Free PMC article. - Connective tissue growth factor (CTGF) in age-related vascular pathologies.
Ungvari Z, Valcarcel-Ares MN, Tarantini S, Yabluchanskiy A, Fülöp GA, Kiss T, Csiszar A. Ungvari Z, et al. Geroscience. 2017 Dec;39(5-6):491-498. doi: 10.1007/s11357-017-9995-5. Epub 2017 Sep 5. Geroscience. 2017. PMID: 28875415 Free PMC article. Review. - A disintegrin and metalloprotease with thrombospondin type I motif 7: a new protease for connective tissue growth factor in hepatic progenitor/oval cell niche.
Pi L, Jorgensen M, Oh SH, Protopapadakis Y, Gjymishka A, Brown A, Robinson P, Liu C, Scott EW, Schultz GS, Petersen BE. Pi L, et al. Am J Pathol. 2015 Jun;185(6):1552-63. doi: 10.1016/j.ajpath.2015.02.008. Epub 2015 Apr 2. Am J Pathol. 2015. PMID: 25843683 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Miscellaneous